微生物塑料工厂的工艺设计：聚羟基脂肪酸酯的代谢工程
Process design for microbial plastic factories: metabolic engineering of polyhydroxyalkanoates.
作者信息
Aldor Ilana S, Keasling Jay D
机构信息
Department of Chemical Engineering, 201 Gilman Hall, University of California, Berkeley, CA 94720-1462, USA.
出版信息
Curr Opin Biotechnol. 2003 Oct;14(5):475-83. doi: 10.1016/j.copbio.2003.09.002.
Implementing several metabolic engineering strategies, either individually or in combination, it is possible to construct microbial plastic factories to produce a variety of polyhydroxyalkanoate (PHA) biopolymers with desirable structures and material properties. Approaches include external substrate manipulation, inhibitor addition, recombinant gene expression, host cell genome manipulation and, most recently, protein engineering of PHA biosynthetic enzymes. In addition, mathematical models and molecular methods can be used to elucidate metabolically engineered systems and to identify targets for performance improvement.
实施几种代谢工程策略，无论是单独实施还是组合实施，都有可能构建微生物塑料工厂，以生产具有理想结构和材料特性的各种聚羟基脂肪酸酯（PHA）生物聚合物。方法包括外部底物操纵、添加抑制剂、重组基因表达、宿主细胞基因组操纵，以及最近的PHA生物合成酶的蛋白质工程。此外，数学模型和分子方法可用于阐明代谢工程系统，并确定性能改进的目标。
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