Lee Li Kim, Roth Charles M
Department of Chemical and Biochemical Engineering, Rutgers University, 98 Brett Road, Piscataway, NJ 08854, USA.
Curr Opin Biotechnol. 2003 Oct;14(5):505-11. doi: 10.1016/s0958-1669(03)00115-0.
Antisense technology is finding increasing application not only in clinical development, but also for cellular engineering. Several types of antisense methods (e.g. antisense oligonucleotides, antisense RNA and small interfering RNA) can be used to inhibit the expression of a target gene. These antisense methods are being used as part of metabolic engineering strategies to downregulate enzymes controlling undesired pathways with regard to product formation. In addition, they are beginning to be utilized to control cell phenotype in tissue engineering constructs. As improved methods for antisense effects that can be externally regulated emerge, these approaches are likely to find increased application in cellular engineering applications.
反义技术不仅在临床开发中得到越来越多的应用，在细胞工程中也是如此。几种类型的反义方法（如反义寡核苷酸、反义RNA和小干扰RNA）可用于抑制靶基因的表达。这些反义方法正被用作代谢工程策略的一部分，以下调控制与产物形成相关的非期望途径的酶。此外，它们开始被用于控制组织工程构建物中的细胞表型。随着可外部调控的反义效应改进方法的出现，这些方法可能会在细胞工程应用中得到更多应用。
