Li Qiang, Xu Xin, Yang Jianming, Nie Qingjuan, Xian Mo
Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266071, China.
Sheng Wu Gong Cheng Xue Bao. 2008 Oct;24(10):1689-94.
With the rapid development of genetic engineering and metabolic regulation, antisense technology displays its fascination to the world as a mild regulation genetic tool. Compared with other loss-of-function research methods (e.g. gene knockout), antisense technologies have advantages such as low cost, short period, and easy operation. It has been increasingly used in bacterial metabolic regulation as a powerful genetic tool. This review briefly summarized the latest progress and problems in antisense technologies that are recently used in metabolic engineering of bacteria, and compares the advantages and disadvantages of these technologies.
随着基因工程和代谢调控的快速发展，反义技术作为一种温和的调控基因工具向世界展现出它的魅力。与其他功能缺失研究方法（如基因敲除）相比，反义技术具有成本低、周期短、操作简便等优点。它作为一种强大的基因工具已越来越多地应用于细菌代谢调控。本文简要总结了细菌代谢工程中近期使用的反义技术的最新进展和存在的问题，并比较了这些技术的优缺点。
