Izutsu K T, Smuckler E A
Am J Pathol. 1978 Jan;90(1):145-58.
Calcium adenosine triphosphatase (Ca-ATPase) activity in rat liver plasma membrane fractions prepared by zonal centrifugation was studied for sensitivity to carbon tetrachloride (CCl4). Levels of Ca-ATPase activity in such membrane fractions from animals given CCl4 by gastric intubation were no different from those in plasmalemmal fractions obtained from control rats. When the fractions were incubated in vitro, however, this enzyme activity was inhibited by the presence of CCl4 in a dose-dependent manner. Moreover, inhibition of Ca-ATPase could be reversed by the removal of CCl4. These results would explain the observed increase in hepatic calcium content following the administration of CCl4 if the Ca-ATPase were capable of actively extruding calcium and if this extrusion mechanism proved sensitive to CCl4 present on or about the hepatocytes.
通过区带离心制备的大鼠肝细胞膜组分中的钙三磷酸腺苷酶（Ca - ATP酶）活性，针对其对四氯化碳（CCl₄）的敏感性进行了研究。经胃插管给予CCl₄的动物的此类膜组分中的Ca - ATP酶活性水平，与从对照大鼠获得的质膜组分中的活性水平没有差异。然而，当这些组分在体外孵育时，该酶活性受到CCl₄存在的剂量依赖性抑制。此外，去除CCl₄可逆转Ca - ATP酶的抑制作用。如果Ca - ATP酶能够主动排出钙，并且如果这种排出机制对存在于肝细胞上或其周围的CCl₄敏感，那么这些结果将解释在给予CCl₄后观察到的肝钙含量增加的现象。
