Lee T M, Chang E, Yang C Y
Institute of Oral Medicine, National Cheng Kung University, Tainan 701, Taiwan.
Biomaterials. 2004 Jan;25(1):23-32. doi: 10.1016/s0142-9612(03)00465-4.
This study examined the cell attachment and proliferation of neonatal rat calvarial osteoblasts on Ti6Al4V alloy as affected by the surface modifications. The modifications could alter simultaneously the surface chemistries of the alloy (elemental difference of Ti, Al, V, Cu and Ni about 300-600mum thick examined by EDS) as well as the XPS nano-surface characteristics of oxides on the metal surface (chemistries of oxides, amphoteric OH group adsorbed on oxides, and oxide thickness). Three materials including two from modifications and a control were examined. It is argued that a slight change of the nano-surface characteristics of oxides as a result of the modifications neither alters the in vitro capability of Ca and P ion adsorption nor affects the metal ion dissolution behavior of the alloy. This implies that any influence on the cytocompatibility of the materials should only be correlated to the effect of surface chemistries of the alloy and the associated metal ion dissolution behavior of the alloy. The experimental results suggest that the cell response of neonatal rat calvarial osteoblasts on the Ti6Al4V alloy should neither be affected by the variation of surface chemistries of the alloy in a range studied.
本研究考察了表面改性对新生大鼠颅骨成骨细胞在Ti6Al4V合金上的细胞黏附及增殖的影响。这些改性能够同时改变合金的表面化学性质（通过能谱仪检测Ti、Al、V、Cu和Ni在约300 - 600μm厚度范围内的元素差异）以及金属表面氧化物的XPS纳米表面特征（氧化物的化学性质、吸附在氧化物上的两性OH基团以及氧化物厚度）。研究了三种材料，其中两种是经过改性的，一种是对照材料。有人认为，改性导致的氧化物纳米表面特征的轻微变化既不会改变体外钙和磷离子的吸附能力，也不会影响合金的金属离子溶解行为。这意味着对材料细胞相容性的任何影响都应仅与合金表面化学性质的影响以及合金相关的金属离子溶解行为相关。实验结果表明，在所研究的范围内，新生大鼠颅骨成骨细胞对Ti6Al4V合金的细胞反应不应受到合金表面化学性质变化的影响。
