Chou Wan-Ching, Wang Rex C-C, Liu Cheng, Yang Chyun-Yu, Lee Tzer-Min
Institute of Manufacturing Information and Systems, National Cheng Kung University, Tainan 701, Taiwan.
Hyperbaric Oxygen Therapy Center and Division of Plastic Surgery, Chi Mei Medical Center, Tainan 710, Taiwan.
Materials (Basel). 2017 Oct 25;10(11):1223. doi: 10.3390/ma10111223.
The sand-blasting and acid etching (SLA) method can fabricate a rough topography for mechanical fixation and long-term stability of titanium implant, but can not achieve early bone healing. This study used two kinds of plasma treatments (Direct-Current and Radio-Frequency plasma) to modify the SLA-treated surface. The modification of plasma treatments creates respective power range and different content functional OH groups. The results show that the plasma treatments do not change the micron scale topography, and plasma-treated specimens presented super hydrophilicity. The X-ray photoelectron spectroscopy (XPS)-examined result showed that the functional OH content of the RF plasma-treated group was higher than the control (SLA) and DC treatment groups. The biological responses (protein adsorption, cell attachment, cell proliferation, and differentiation) promoted after plasma treatments, and the cell responses, have correlated to the total content of amphoteric OH groups. The experimental results indicated that plasma treatments can create functional OH groups on SLA-treated specimens, and the RF plasma-treated SLA implant thus has potential for achievement of bone healing in early stage of implantation.
喷砂酸蚀（SLA）法可为钛种植体制造用于机械固定和长期稳定性的粗糙表面形貌，但无法实现早期骨愈合。本研究采用两种等离子体处理（直流和射频等离子体）对经SLA处理的表面进行改性。等离子体处理的改性产生了各自的功率范围和不同含量的功能性羟基。结果表明，等离子体处理未改变微米级表面形貌，且经等离子体处理的试样呈现出超亲水性。X射线光电子能谱（XPS）检测结果显示，射频等离子体处理组的功能性羟基含量高于对照组（SLA）和直流处理组。等离子体处理后生物反应（蛋白质吸附、细胞黏附、细胞增殖和分化）得到促进，且细胞反应与两性羟基的总含量相关。实验结果表明，等离子体处理可在经SLA处理的试样上产生功能性羟基，因此经射频等离子体处理的SLA种植体在植入早期具有实现骨愈合的潜力。
