成纤维细胞对27纳米高聚物相分离纳米拓扑结构的快速黏附。

Rapid fibroblast adhesion to 27nm high polymer demixed nano-topography.

作者信息

Dalby M J, Giannaras D, Riehle M O, Gadegaard N, Affrossman S, Curtis A S G

机构信息

Centre for Cell Engineering, Institute of Biomedical and Life Sciences, University of Glasgow, Joseph Black Building, Glasgow, G12 8QQ, UK.

出版信息

Biomaterials. 2004 Jan;25(1):77-83. doi: 10.1016/s0142-9612(03)00475-7.

DOI:10.1016/s0142-9612(03)00475-7

PMID:14580911

Abstract

It is well known that many cell types react strongly to micro-topography. It is rapidly becoming clear than cells will also react to nano-topography. Polymer demixing is a rapid and low-cost chemical method of producing nano-topography. This manuscript investigates human fibroblast response to 27nm high nano-islands produced by polymer demixing. Cell spreading, cytoskeleton, focal adhesion and Rac localisation were studied. The results showed that an initial rapid adhesion and cytoskeletal formation on the islands at 4 days of culture gave way to poorly formed contacts and vimentin cytoskeleton at 30 days of culture.

摘要

众所周知，许多细胞类型对微观形貌有强烈反应。越来越明显的是，细胞也会对纳米形貌产生反应。聚合物相分离是一种快速且低成本的产生纳米形貌的化学方法。本手稿研究了人类成纤维细胞对聚合物相分离产生的27纳米高纳米岛的反应。研究了细胞铺展、细胞骨架、粘着斑和Rac定位。结果表明，培养4天时细胞在这些岛上最初的快速粘附和细胞骨架形成，在培养30天时让位于形成不良的接触和波形蛋白细胞骨架。