Zuppa Athena F, Shi Heng, Adamson Peter C
Division of Clinical Pharmacology and Therapeutics, Department of Pediatrics, The Children's Hospital of Philadelphia, Abramson Research Center, Suite 907, 3615 Civic Center Blvd., Philadelphia, PA 19104-4318, USA.
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Nov 5;796(2):293-301. doi: 10.1016/j.jchromb.2003.08.014.
Esmolol is a very short-acting beta-blocker commonly used in critically ill patients. To study the pharmacokinetics of esmolol in pediatric patients, a liquid chromatography-electrospray mass spectrometry (LC-MS) method to quantitate esmolol concentration in plasma was developed. Following methylene chloride extraction from 200 microl aliquots of plasma containing internal standard and reconstitution in 0.05% formic acid, 10 microl are injected onto column, eluted using a methanol/formic acid gradient over 15 min, and monitored with selected ion recording at 296.2 and 282.2. The assay is linear between 2 and 1000 ng/ml. The intra-day and inter-day coefficients of variation are less than 8 and 10%, respectively.
艾司洛尔是一种常用于危重症患者的超短效β受体阻滞剂。为研究艾司洛尔在儿科患者中的药代动力学，开发了一种用于定量血浆中艾司洛尔浓度的液相色谱-电喷雾质谱（LC-MS）方法。从含有内标的200微升血浆等分试样中用二氯甲烷萃取，然后在0.05%甲酸中复溶，取10微升进样到色谱柱上，使用甲醇/甲酸梯度在15分钟内洗脱，并在296.2和282.2处用选择离子记录进行监测。该测定法在2至1000纳克/毫升之间呈线性。日内和日间变异系数分别小于8%和10%。
