Wrona Danuta, Jurkowski Marek, Luszawska Danuta, Tokarski Juliusz, Trojniar Weronika
Department of Animal Physiology, University of Gdańsk, Kładki 24, Gdańsk 80-822, Poland.
Brain Behav Immun. 2003 Dec;17(6):453-61. doi: 10.1016/s0889-1591(03)00065-5.
Individual variability in the central control of the cellular immune responses is the main subject of the study. Previously, it was found that destruction of the lateral hypothalamus (LH) produced long-term depression of the cytotoxicity of NK cells (NKCC) and their number (LGL). In the present experiment we compared changes in the peripheral blood NKCC, LGL number, as well as leukocyte and lymphocyte number, their mitogenic activity and plasma corticosterone level evoked by electrolytic LH lesions in rats which were categorized as either high (HR) and low (LR) responders according to their locomotor response to a new environment. It was found that: (1) before the lesion NKCC (measured by 51Cr release assay) was higher in the HRs than in LRs; (2) LH damage caused a drop in NKCC and LGL number (21st postlesion day) preceded by a transient enhancement (5th postlesion day) significant for HRs only. As a result of a greater decrease in the HRs than LRs the baseline differences between groups disappeared by 21st postlesion day; (3) NKCC and LGL depression was not accompanied by changes in lytic activity of a single NK cell (agarose assay) which indicates that NKCC decrease concerned the population level and was dependent on LGL redistribution and/or recycling rate; (4) on the 21st postlesion day there was a significant leuko- and lymphopenia in the lesioned groups both HRs and LRs; (5) proliferative lymphocyte response to PWM (colorimetric assay) and plasma corticosterone level were not affected either by the motility level or by the lesion. The results emphasize the importance of individual differences in behavioral reactivity for NKCC regulation and a possible involvement of LH in the mechanism which connects high locomotor activity with stimulation of NKCC.
细胞免疫反应中枢控制中的个体差异是本研究的主要主题。先前发现，损毁下丘脑外侧区（LH）会导致自然杀伤细胞（NK）细胞毒性（NKCC）及其数量（LGL）长期降低。在本实验中，我们比较了根据大鼠对新环境的运动反应分为高反应者（HR）和低反应者（LR）的两组大鼠，经电解损毁LH后外周血NKCC、LGL数量、白细胞和淋巴细胞数量、它们的促有丝分裂活性以及血浆皮质酮水平的变化。结果发现：（1）在损毁前，HR组的NKCC（通过51Cr释放试验测量）高于LR组；（2）LH损伤导致NKCC和LGL数量下降（损伤后第21天），在此之前有一个短暂的增强（损伤后第5天），且仅在HR组中显著。由于HR组的下降幅度大于LR组，两组之间的基线差异在损伤后第21天消失；（3）NKCC和LGL的降低并未伴随着单个NK细胞的溶解活性变化（琼脂糖试验），这表明NKCC的降低与群体水平有关，并且依赖于LGL的重新分布和/或循环率；（4）在损伤后第21天，损伤组（HR组和LR组）均出现明显的白细胞减少和淋巴细胞减少；（5）淋巴细胞对PWM的增殖反应（比色法）和血浆皮质酮水平均未受运动水平或损伤的影响。这些结果强调了行为反应性个体差异对NKCC调节的重要性，以及LH可能参与了将高运动活性与NKCC刺激联系起来的机制。
