Wrona D, Jurkowski M K, Trojniar W, Staszewska M, Tokarski J
Department of Animal Physiology, University of Gdańsk, Poland.
J Neuroimmunol. 1994 Nov;55(1):45-54. doi: 10.1016/0165-5728(94)90145-7.
Bilateral electrolytic lesions of the lateral hypothalamic (LH) area in Wistar rats result in a time-dependent blood NK cytotoxicity changes as measured by the 51Cr-release (for entire cell population) and agarose (for a single-cell) assays. NK activity against YAC-1 and K-562 cells shifts from depression through enhancement to another depression on the 2nd, 5th and 21st post-lesion day, respectively, as compared to both LH sham-operated animals and the pre-lesion baselines. This effect is not attributable to malnutrition and dehydration resulting from ingestive impairments evoked by LH lesions. No significant change in NK cytotoxicity was found after destruction of the medial hypothalamus (MH). The results indicate that LH, under normal conditions, which may be considered as a dynamogenic and stressogenic hypothalamic area is essential for proper regulations of NK cytotoxicity at both population and single-cell level.
对Wistar大鼠双侧下丘脑外侧（LH）区域进行电解损伤，会导致血液中自然杀伤细胞（NK）细胞毒性随时间变化，这通过51铬释放法（针对整个细胞群体）和琼脂糖法（针对单个细胞）进行测定。与LH假手术动物和损伤前基线相比，针对YAC - 1和K - 562细胞的NK活性在损伤后第2天、第5天和第21天分别从抑制转变为增强，再转变为另一次抑制。这种效应并非由LH损伤引起的摄食障碍导致的营养不良和脱水所致。破坏下丘脑内侧（MH）后，未发现NK细胞毒性有显著变化。结果表明，在正常条件下，LH可被视为一个产生动力和应激的下丘脑区域，对于在群体和单细胞水平上正确调节NK细胞毒性至关重要。
