Wrona Danuta, Trojniar Weronika
Department of Animal Physiology, University of Gdansk, 80-822 Gdansk, Poland.
J Neuroimmunol. 2003 Aug;141(1-2):20-9. doi: 10.1016/s0165-5728(03)00214-5.
Previously, we found that in rats coagulation of the lateral hypothalamus (LH) caused depression of the peripheral blood natural killer cell cytotoxicity (NKCC) and the number of large granular lymphocytes (LGL). In the present work, we have tested the effects on both spleen and blood NKCC of acute (1 day) and chronic (21 days) electrical stimulation of LH, and LGL number in conscious, freely behaving animals. Five groups of male Wistar rats were used: LH stimulated (n=22), thalamic (Thal) stimulated control (n=4), operated but non-stimulated LH sham controls (n=7), non-operated normal control group (n=8) and spleen baseline group (n=10). Chronic stimulation of LH caused significant augmentation of NKCC (51Cr-release assay) and LGL number (a morphological method), more pronounced in the spleen than in the peripheral blood. Rats responding to LH stimulation with feeding showed a slightly greater effect than those responding with a locomotor reaction. The observed effects were anatomically specific as no influence of Thal stimulation or the sham procedure was found. The results are discussed in terms of the involvement of LH in reward phenomena and the hormonal control of the immune system.
此前，我们发现，对大鼠下丘脑外侧区（LH）进行凝血会导致外周血自然杀伤细胞细胞毒性（NKCC）以及大颗粒淋巴细胞（LGL）数量降低。在本研究中，我们测试了对清醒、自由活动动物的LH进行急性（1天）和慢性（21天）电刺激对脾脏和血液NKCC以及LGL数量的影响。使用了五组雄性Wistar大鼠：LH刺激组（n = 22）、丘脑（Thal）刺激对照组（n = 4）、手术但未刺激的LH假手术对照组（n = 7）、未手术的正常对照组（n = 8）以及脾脏基线组（n = 10）。对LH进行慢性刺激会导致NKCC（51Cr释放试验）以及LGL数量（一种形态学方法）显著增加，在脾脏中的增加比在外周血中更明显。对LH刺激有进食反应的大鼠比有运动反应的大鼠效果略好。观察到的效应具有解剖学特异性，因为未发现Thal刺激或假手术有影响。从LH参与奖赏现象以及免疫系统的激素控制方面对结果进行了讨论。
