一种类IQGAP蛋白与Cdc42共同参与海胆卵中的肌动蛋白组装。
An IQGAP-like protein is involved in actin assembly together with Cdc42 in the sea urchin egg.
作者信息
Nishimura Yukako, Mabuchi Issei
机构信息
Division of Biology, Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo.
出版信息
Cell Motil Cytoskeleton. 2003 Dec;56(4):207-18. doi: 10.1002/cm.10146.
We isolated a gene homologous to human cdc42 (ucdc42) from a sea urchin cDNA library. The GTPgammaS-bound UCdc42 induced actin assembly in sea urchin egg extract. Proteins that are involved in this actin assembly system were searched using UCdc42-bound agarose beads. A 180-kDa protein (p180), which showed a homology to human IQGAPs, bound to the GTPgammaS-UCdc42 beads. Immunodepletion of p180 from the sea urchin egg extract abolished this actin assembly on the UCdc42 beads. Immunofluorescent localization of p180 was similar to that of the actin cytoskeleton in the egg cortex and it was concentrated in the cleavage furrow during cytokinesis. A possible role of p180 in actin assembly is discussed.
我们从海胆cDNA文库中分离出一个与人类cdc42（ucdc42）同源的基因。结合GTPγS的UCdc42在海胆卵提取物中诱导肌动蛋白组装。使用结合UCdc42的琼脂糖珠搜索参与该肌动蛋白组装系统的蛋白质。一种与人类IQGAPs具有同源性的180 kDa蛋白质（p180）与结合GTPγS的UCdc42珠结合。从海胆卵提取物中免疫去除p180消除了在UCdc42珠上的这种肌动蛋白组装。p180的免疫荧光定位与卵皮质中肌动蛋白细胞骨架的定位相似，并且在胞质分裂期间集中在分裂沟中。讨论了p180在肌动蛋白组装中的可能作用。
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