Feist Iliria S, De Micheli Giorgio, Carneiro Silvia R S, Eduardo Carlos P, Miyagi Sueli, Marques Márcia M
Department of Periodontics, School of Dentistry, University of São Paulo, São Paulo, Brazil.
J Periodontol. 2003 Sep;74(9):1368-75. doi: 10.1902/jop.2003.74.9.1368.
The application of Er:YAG laser irradiation, approved in 1997 to be used on dental hard tissues, has been investigated for periodontal therapy. The aim of this study was to analyze the biocompatibility of root surfaces treated by Er:YAG laser.
Adhesion and growth of cultured human gingival fibroblasts on root surfaces treated by either irradiation with Er:YAG laser or curet were compared. Thirty single-rooted teeth extracted because of periodontal disease were used. Calculus deposits on all experimental surfaces were removed, and the teeth were divided into three groups according to the applied treatment: group A, root planing with Gracey curet no. 3/4; group B, two irradiations with laser (60 mJ/pulse, 10 Hz; 10" each with 10-second interval, 3 J/cm2); group C, two irradiations with laser (100 mJ/pulse, 10 Hz; 10" each with 10-second interval, 5 J/cm2). Fragments (5 mm x 6 mm) were obtained from the experimental surfaces. Then, 1 x 10(3) cells were seeded on the top of each fragment. One, 2, and 3 days after seeding the specimens were prepared for scanning electron microscopy analysis, and the cells on the electronmicrographs were counted. The data obtained in triplicate were statistically compared by the Kruskall-Wallis test complemented by the Dunn test (P < or = 0.05).
Human gingival fibroblasts adhered to and grew on all treated surfaces. Group B presented a significantly higher cell count than did the other two groups at days 1 and 2. Three days after seeding the cultured fibroblasts of groups A and B reached total confluence. The cell count of group B was significantly higher than that of group C.
The surfaces treated with 60 mJ/pulse Er:YAG laser irradiation promoted faster adhesion and growth than surfaces treated with either root planing or 100 mJ/pulse Er:YAG laser irradiation.
铒钇铝石榴石（Er:YAG）激光照射于1997年被批准用于牙科硬组织，目前已被研究用于牙周治疗。本研究的目的是分析经Er:YAG激光处理的牙根表面的生物相容性。
比较培养的人牙龈成纤维细胞在经Er:YAG激光照射或刮治处理的牙根表面上的黏附及生长情况。使用因牙周疾病而拔除的30颗单根牙。清除所有实验表面的牙结石沉积物，根据所应用的处理方法将牙齿分为三组：A组，用3/4号格雷斯刮治器进行根面平整；B组，两次激光照射（60 mJ/脉冲，10 Hz；每次10秒，间隔10秒，3 J/cm²）；C组，两次激光照射（100 mJ/脉冲，10 Hz；每次10秒，间隔10秒，5 J/cm²）。从实验表面获取碎片（5 mm×6 mm）。然后，将1×10³个细胞接种到每个碎片顶部。接种后1、2和3天，制备标本用于扫描电子显微镜分析，并对电子显微镜照片上的细胞进行计数。一式三份获得的数据通过Kruskal-Wallis检验并辅以Dunn检验进行统计学比较（P≤0.05）。
人牙龈成纤维细胞在所有处理过的表面上均能黏附并生长。在第1天和第2天，B组的细胞计数显著高于其他两组。接种3天后，A组和B组的培养成纤维细胞达到完全汇合。B组的细胞计数显著高于C组。
与根面平整或100 mJ/脉冲Er:YAG激光照射处理的表面相比，60 mJ/脉冲Er:YAG激光照射处理的表面促进了更快的黏附及生长。
