Yamaguchi H, Kobayashi K, Osada R, Sakuraba E, Nomura T, Arai T, Nakamura J
Department of Periodontics and Endodontics, Tsurumi University School of Dental Medicine, Yokohama, Japan.
J Periodontol. 1997 Dec;68(12):1151-5. doi: 10.1902/jop.1997.68.12.1151.
The application of erbium:YAG laser (Er:YAG) irradiation has been investigated for periodontal therapy. The purpose of this study was to evaluate the effects of Er:YAG laser irradiation on root surfaces using a scanning electron microscope (SEM) and to determine the laser's ability to remove lipopolysaccharides (LPS). Infrared spectrophotometry was used to investigate the effects of the laser on LPS applied to root dentin pellets. Premolars extracted for orthodontic reasons were prepared for this study. The crowns were resected below the cemento-enamel junction, longitudinally sectioned, and the contents of the pulp chamber were removed. Then 15 root tips (5 x 5 x 1 mm) were classified into 3 groups of 5 each as follows: group 1, tips without any treatment; group 2, planed tips with the cement layers left untouched; and group 3, planed until the dentin surface was disclosed. The center of each specimen was used as the experimental irradiated area and the peripheral area served as a control. The quantity of vapor delivered by Er:YAG laser was highly increased, and the irradiated areas displayed little morphogenetic changes. The lyophilized sample LPS 0111 B4 from E. coli was then mixed with potassium bromide and pressed into a tablet, which was examined at 4,000-650 Kayser. The lyophilized LPS had a peak at 2.94 microns. LPS on the root dentin pellets was cleared away as much as possible by 150 washings in pyrogen-free water using an ultrasonic cleaner. Five microliters of 24 EU LPS solution was dropped on the root dentin pellets, which were then irradiated by the Er:YAG laser, and washed using the ultrasonic cleaner in pyrogen-free water. The amount of the extracted LPS solution was determined by spectrophotometer at 405 nm. The Er:YAG laser could remove 83.1% of the LPS. This study suggests that Er:YAG laser irradiation might be useful for root conditioning in periodontal therapy. However, clinical testing is necessary to establish what, if any, utility the Er:YAG laser has as a part of periodontal therapy.
铒钇铝石榴石激光（Er:YAG）照射在牙周治疗中的应用已得到研究。本研究的目的是使用扫描电子显微镜（SEM）评估Er:YAG激光照射对牙根表面的影响，并确定该激光去除脂多糖（LPS）的能力。采用红外分光光度法研究激光对应用于牙根牙本质颗粒的LPS的影响。本研究使用因正畸原因拔除的前磨牙。将牙冠在牙骨质-釉质界下方切除，纵向切片，并去除髓腔内容物。然后将15个根尖（5×5×1毫米）分为3组，每组5个，如下所示：第1组，未进行任何处理的根尖；第2组，刮平的根尖，保留牙骨质层；第3组，刮平直至露出牙本质表面。每个标本的中心用作实验照射区域，周边区域用作对照。Er:YAG激光产生的蒸汽量显著增加，照射区域显示出微小的形态发生变化。然后将来自大肠杆菌的冻干样品LPS 0111 B4与溴化钾混合并压制成片剂，在4000 - 650波数下进行检测。冻干的LPS在2.94微米处有一个峰值。使用超声波清洗器在无热原水中对牙根牙本质颗粒上的LPS进行150次洗涤，尽可能将其清除。将5微升24 EU的LPS溶液滴在牙根牙本质颗粒上，然后用Er:YAG激光照射，并用超声波清洗器在无热原水中洗涤。通过分光光度计在405纳米处测定提取的LPS溶液的量。Er:YAG激光可去除83.1%的LPS。本研究表明，Er:YAG激光照射可能对牙周治疗中的牙根预处理有用。然而，需要进行临床试验以确定Er:YAG激光作为牙周治疗一部分的实际效用（如果有的话）。
