Utsugisawa K, Sano M
Department of Neurology, Iwate Medical University.
Rinsho Shinkeigaku. 1992 May;32(5):474-8.
Interleukin-2 (IL-2) production by peripheral blood mononuclear cell (PBM) and the frequency of surface IgG+ B cells (%) were studied in 17 patients with myasthenia gravis (MG) and 12 controls. Culture supernatants from phytohemagglutinin (PHA)-stimulated peripheral blood mononuclear cell from patients with MG and controls were added to IL-2 dependent proliferative cultures (CTLL-2). Following 24 hr of culture, 3H-Tdr was added and cultured for an additional 6 hr. Cultures were harvested and 3H-Tdr incorporation was determined. In comparison with controls we found significantly increased synthesis of IL-2 in patients with MG (p < 0.05). Highest synthesis was found in severe cases and there were highly significant correlation between IL-2 synthesis and clinical severity score (R = 0.85, p < 0.05). These findings suggest that the ability of IL-2 synthesis by PBM may reflect severity of patients with MG. B cells expressing surface IgG, considered to be differentiated B cells, were less frequent in severe cases (R = -0.67, p < 0.05). It may be that the B cells with further differentiation are transformed into plasma cells expressing other markers such as PCA-1 in severe patients with MG.
对17例重症肌无力（MG）患者和12例对照者的外周血单个核细胞（PBM）产生白细胞介素-2（IL-2）的情况以及表面IgG+B细胞的频率（%）进行了研究。将MG患者和对照者经植物血凝素（PHA）刺激的外周血单个核细胞的培养上清液加入到依赖IL-2增殖的培养物（CTLL-2）中。培养24小时后，加入3H-Tdr并再培养6小时。收获培养物并测定3H-Tdr掺入量。与对照者相比，我们发现MG患者的IL-2合成显著增加（p<0.05）。重症患者中IL-2合成最高，且IL-2合成与临床严重程度评分之间存在高度显著相关性（R=0.85，p<0.05）。这些发现表明，PBM合成IL-2的能力可能反映MG患者的严重程度。表达表面IgG的B细胞被认为是分化的B细胞，在重症患者中频率较低（R=-0.67，p<0.05）。在重症MG患者中，进一步分化的B细胞可能会转变为表达其他标志物如PCA-1的浆细胞。
