Casas J, Partal Y, Llosá J, Leiva J, Navarro J M, de la Rosa M
Servicio de Microbiología, Hospital Virgen de las Nieves, Granada.
Enferm Infecc Microbiol Clin. 1994 Dec;12(10):497-500.
The ability of in vitro and in vivo detection of Brucella spp. with the Bact/Alert system was studied.
Three strains of Brucella melitensis and two of Brucella abortus were used. Different dilutions of the five strains were performed in trypticase soy broth (TSB), achieving concentrations of 1 cfu/ml, 5 cfu/ml, 10 cfu/ml and 100 cfu/ml. Ten ml of each dilution and strain were inoculated into 5 aerobic bottles Bact/Alert and 5 biphasic Hemóline bottles. Furthermore, over a 9 month period, 8,216 bottles of Bact/Alert bottles from hospitalized patients and from the emergency department were processed in the authors' laboratory.
The mean detection time for Brucella growth was from 2 to 3 days with the Bact/Alert system, and 14 days in the biphasic bottles. Former bottles processed in the authors' laboratory, 11 aerobic bottles belonged to 5 patients in whom brucelosis was confirmed by bloodculture. The Bact/Alert system detected Brucella melitensis in only on bottle at 2.9 days of incubation. In 7 bottles Bact/Alert detected B. melitensis by a blind pass of these bottles at 10 to 20 days of incubation.
These results suggest that the Bact/Alert system does not totally solve the diagnosis of brucellosis. Blind passes of the bloodcultures are required.
研究了使用Bact/Alert系统进行体外和体内检测布鲁氏菌属的能力。
使用了三株羊种布鲁氏菌和两株牛种布鲁氏菌。将这五株菌在胰蛋白胨大豆肉汤（TSB）中进行不同稀释，得到浓度为1 cfu/ml、5 cfu/ml、10 cfu/ml和100 cfu/ml的菌液。将每种稀释度和菌株的10 ml菌液接种到5个Bact/Alert需氧瓶和5个双相Hemóline瓶中。此外，在9个月的时间里，作者实验室对来自住院患者和急诊科的8216个Bact/Alert瓶进行了处理。
使用Bact/Alert系统，布鲁氏菌生长的平均检测时间为2至3天，而在双相瓶中为14天。在作者实验室处理的先前的瓶子中，11个需氧瓶属于5名患者，这些患者的布鲁氏菌病通过血培养得到确诊。Bact/Alert系统仅在一个培养2.9天的瓶子中检测到羊种布鲁氏菌。在7个瓶子中，Bact/Alert在培养10至20天时通过对这些瓶子的盲传检测到羊种布鲁氏菌。
这些结果表明，Bact/Alert系统并不能完全解决布鲁氏菌病的诊断问题。需要对血培养进行盲传。
