Coates T D
Department of Pediatrics, Childrens Hospital of Los Angeles, University of Southern California, School of Medicine 90027.
Comput Methods Programs Biomed. 1992 Jul;38(2-3):177-92. doi: 10.1016/0169-2607(92)90085-l.
We have established a computerized system for quantification of human neutrophil motility using a 48-well chemotaxis assay. The software is primarily written in the Unix C-shell and is designed to integrate with standard statistical and graphics packages and permit analysis either under Unix or MS-DOS. We demonstrate how simple image analysis techniques may be used to count neutrophils that have traversed a polycarbonate filter. Methods of optical optimization, cell counting and integration with the Statistical Analysis System (SAS) are presented.
我们已经建立了一个使用48孔趋化性分析来定量人类中性粒细胞运动性的计算机系统。该软件主要用Unix C-shell编写，旨在与标准统计和图形软件包集成，并允许在Unix或MS-DOS下进行分析。我们展示了如何使用简单的图像分析技术来计数穿过聚碳酸酯滤膜的中性粒细胞。本文介绍了光学优化、细胞计数以及与统计分析系统（SAS）集成的方法。
