Coates T D, Harman J T, McGuire W A
Comput Methods Programs Biomed. 1985 Dec;21(3):195-202. doi: 10.1016/0169-2607(85)90004-5.
We have developed a computerized system for the quantification of video images of cell migration patterns obtained in the chemotaxis under agarose assay. This system allows either manual or automatic measurement of standard parameters of chemotaxis, as well as quantification of three new parameters which reflect mean cell movement and chemokinesis. The parameters of mean cell population movement are not obtainable by the traditional analysis of the chemotaxis under agarose assay. Our system operates on an inexpensive microcomputer, is easy to use and provides data output in a format that is directly transferable to standard statistical packages.
我们开发了一种计算机化系统，用于对在琼脂糖下趋化性实验中获得的细胞迁移模式的视频图像进行定量分析。该系统允许手动或自动测量趋化性的标准参数，以及对反映平均细胞运动和化学动力学的三个新参数进行定量分析。平均细胞群体运动的参数无法通过传统的琼脂糖下趋化性实验分析获得。我们的系统在一台廉价的微型计算机上运行，易于使用，并以可直接传输到标准统计软件包的格式提供数据输出。
