A microcomputer-based program for video analysis of chemotaxis under agarose.

We have developed a computerized system for the quantification of video images of cell migration patterns obtained in the chemotaxis under agarose assay. This system allows either manual or automatic measurement of standard parameters of chemotaxis, as well as quantification of three new parameters which reflect mean cell movement and chemokinesis. The parameters of mean cell population movement are not obtainable by the traditional analysis of the chemotaxis under agarose assay. Our system operates on an inexpensive microcomputer, is easy to use and provides data output in a format that is directly transferable to standard statistical packages.