Schutzner W, Fanali S
Istituto di Cromatografia del C.N.R., Monterotondo Scalo, Roma, Italy.
Electrophoresis. 1992 Sep-Oct;13(9-10):687-90. doi: 10.1002/elps.11501301146.
Cyclodextrins added to the background electrolyte are shown to be useful for the resolution of racemic compounds in their enantiomers. Several parameters have to be controlled in order to achieve resolution, e.g., cyclodextrin type, concentration, analyte shape, as well as column temperature. The resolution of nor-epinephrine, epinephrine and isoproterenol in their enantiomers decreased by increasing the column temperature. Octopamine and ketamine have been resolved by supporting the background electrolyte with 2, 6-di-O-methyl-beta-cyclodextrin. In spite of the stronger inclusion-complex of ketamine than octopamine with the modified cyclodextrin its resolution was not satisfactory.
已表明添加到背景电解质中的环糊精可用于拆分外消旋化合物的对映体。为了实现拆分，必须控制几个参数，例如环糊精类型、浓度、分析物形状以及柱温。随着柱温升高，去甲肾上腺素、肾上腺素和异丙肾上腺素对映体的拆分效果降低。通过用2,6-二-O-甲基-β-环糊精支持背景电解质，实现了章鱼胺和氯胺酮的拆分。尽管氯胺酮与修饰环糊精形成的包合物比章鱼胺更强，但其拆分效果并不理想。
