Bronson R A, O'Connor W J, Wilson T A, Bronson S K, Chasalow F I, Droesch K
Department of Obstetrics and Gynecology, Health Sciences Center, SUNY, Stony Brook 11794-8091.
Fertil Steril. 1992 Dec;58(6):1199-204. doi: 10.1016/s0015-0282(16)55569-1.
To test the hypothesis that puberty is a necessary factor in the pathogenesis of autoimmunity to sperm in men with cystic fibrosis (CF), we studied prepubertal and postpubertal males with CF versus an age-matched group of males with type 1 diabetes as controls.
Sera from CF and diabetic males treated at University Hospital, State University of New York, Stony Brook, were tested by indirect immunobead binding for antisperm antibodies and by radioimmunoassay for testosterone (T), luteinizing hormone, and follicle-stimulating hormone. The finding of autoantibodies to spermatozoa was correlated with chronological age, as well as with clinical and hormonal pubertal status.
Autoimmunity to sperm, as detected by humoral antisperm antibodies, was documented solely in postpubertal males, as judged by hormonal and clinical criteria. Eighty-three percent of sexually mature CF males and 6.3% (1 of 16) diabetic males exhibited autoantibodies to sperm. These antibodies were only detected when serum T levels were > 8.7 nmol/L (250 ng/dL).
These results suggest that puberty, and presumably, active spermatogenesis is a requirement for the development of autoimmunity to sperm in men with CF.
为了验证青春期是囊性纤维化（CF）男性精子自身免疫发病机制中的一个必要因素这一假说，我们研究了青春期前和青春期后的CF男性，并与年龄匹配的1型糖尿病男性作为对照组。
对在纽约州立大学石溪分校大学医院接受治疗的CF男性和糖尿病男性的血清进行检测，通过间接免疫珠结合法检测抗精子抗体，通过放射免疫分析法检测睾酮（T）、黄体生成素和卵泡刺激素。精子自身抗体的检测结果与实际年龄以及临床和激素青春期状态相关。
根据激素和临床标准判断，仅在青春期后男性中发现了由体液抗精子抗体检测到的精子自身免疫。83%的性成熟CF男性和6.3%（16例中的1例）糖尿病男性表现出精子自身抗体。仅当血清T水平>8.7 nmol/L（250 ng/dL）时才检测到这些抗体。
这些结果表明，青春期以及可能的活跃精子发生是CF男性精子自身免疫发展的必要条件。
