D'Cruz O J, Haas G G, de La Rocha R, Lambert H
Department of Obstetrics and Gynecology, University of Oklahoma Health Sciences Center, Oklahoma City.
Fertil Steril. 1991 Sep;56(3):519-27.
To determine if acquired obstruction of the vas deferens in men with cystic fibrosis (CF) induced the development of antisperm antibodies with genital tract obstruction similar to other men.
Serum antisperm antibodies were assayed by an indirect immunobead test and an indirect immunofluorescence assay. Both homologous (human sperm/human zona) and heterologous (human sperm/zona-free hamster ova) sperm/egg interactions were evaluated in the presence of serum antisperm antibodies from patients with CF.
Cystic Fibrosis Clinic at the University of Oklahoma Health Sciences Center, a tertiary care referral center.
Fifteen CF patients (10 male and 5 female), 3 non-CF antisperm antibody-positive infertile patients (2 male and 1 female), 20 fertile controls (7 males and 13 females), and 9 fertile sperm donors were used.
None.
Serum antisperm antibody levels in patients with CF. In those patients with antisperm antibodies, determine effect of these sperm antibodies on sperm/egg interactions and complement-mediated events.
Sera from 3 (30%) of 10 men with CF demonstrated immunoglobulin (Ig)G, IgA, and/or IgM antisperm antibodies, whereas sera from all 5 CF women and the 20 control sera were negative for antisperm antibodies. The maximal titers for IgG, IgA, and IgM antisperm antibody were 1:8, 192, 1:256, and 1:64, respectively. The immunobead binding, which was restricted to the sperm head and tail-tip or the midpiece and tail-tip, correlated with the indirect immunofluorescence pattern. Antisperm antibody-positive sera from men with CF impaired both the binding and penetration of human zonae and the penetration of hamster ova by human sperm.
Similar to other men with congenital or acquired obstruction of their genital tract, antisperm antibodies may occur in some men with CF. Antisperm antibodies may contribute to immune sperm dysfunction in some men with CF by activated complement-mediated events and interfering with sperm/egg interactions.
确定患有囊性纤维化（CF）的男性后天性输精管梗阻是否会像其他男性一样引发抗精子抗体并导致生殖道梗阻。
采用间接免疫珠试验和间接免疫荧光测定法检测血清抗精子抗体。在存在CF患者血清抗精子抗体的情况下，评估同源（人精子/人透明带）和异源（人精子/无透明带仓鼠卵）精子/卵子相互作用。
俄克拉荷马大学健康科学中心囊性纤维化诊所，一家三级医疗转诊中心。
使用了15名CF患者（10名男性和5名女性）、3名非CF抗精子抗体阳性的不育患者（2名男性和1名女性）、20名生育对照者（7名男性和13名女性）以及9名生育精子捐献者。
无。
CF患者的血清抗精子抗体水平。对于那些有抗精子抗体的患者，确定这些精子抗体对精子/卵子相互作用和补体介导事件的影响。
10名CF男性中有3名（30%）的血清显示出免疫球蛋白（Ig）G、IgA和/或IgM抗精子抗体，而所有5名CF女性的血清以及20份对照血清的抗精子抗体均为阴性。IgG、IgA和IgM抗精子抗体的最大滴度分别为1:8、1:192、1:256和1:64。免疫珠结合仅限于精子头部和尾尖或线粒体和尾尖，与间接免疫荧光模式相关。CF男性抗精子抗体阳性血清损害了人精子与人透明带的结合和穿透以及人精子对仓鼠卵的穿透。
与其他先天性或后天性生殖道梗阻的男性相似，一些CF男性可能会出现抗精子抗体。抗精子抗体可能通过激活补体介导的事件和干扰精子/卵子相互作用，导致一些CF男性出现免疫性精子功能障碍。
