Vazquez-Levin M H, Kupchik G S, Torres Y, Chaparro C A, Shtainer A, Bonforte R J, Nagler H M
Department of Urology, Beth Israel Medical Center, New York, NY 10003, USA.
J Reprod Immunol. 1994 Dec;27(3):199-212. doi: 10.1016/0165-0378(94)90004-3.
Antisperm antibodies are formed as a result of vasal and epididymal obstruction. Fourteen males of different ages (pre-, peri- and post-pubertal) with bilateral congenital vasal agenesis and epididymal obstruction secondary to cystic fibrosis (CF), and seven men with congenital bilateral aplasia of the vas deferens (CBAVD) were evaluated with regard to both the presence and levels of serum antisperm antibodies, and the CF-genotype. While IgA and IgG were not detected among pre- and peri-pubertal CF patients, 4 out of 10 (40%) exhibited IgM binding to sperm tail-tip. Post-pubertal CF patients showed high antisperm antibody (ASA) levels in 3 of the 4 males (75%) evaluated for the three isotypes assayed. ASA were found in 5 of 7 CBAVD patients (71%); IgG (n = 3) and IgM (n = 4) were found to be the predominant isotypes bound to sperm tail-tip. CF-genotype analysis revealed two pre-pubertal patients with the DeltaF508/DeltaF508 CF-genotype and a positive ASA response, thus suggesting an earlier or more severe blockage. In addition, the two CBAVD patients found to have a ?/? CF-genotype on the initial screening did not have ASA. The altered antigenicity of sperm associated with initiation of spermatogenesis appears to modify the antisperm antibody isotypes. Further studies on a larger number of patients may allow for a better understanding of the ASA response, as well as a better understanding of a possible phenotype/genotype association between the CF-genotype and the immunologic response.
抗精子抗体是由输精管和附睾梗阻形成的。对14名不同年龄（青春期前、青春期前后和青春期后）的男性进行了评估，这些男性患有双侧先天性输精管缺如以及继发于囊性纤维化（CF）的附睾梗阻，还有7名患有先天性双侧输精管发育不全（CBAVD）的男性，评估内容包括血清抗精子抗体的存在情况和水平以及CF基因型。青春期前和青春期前后的CF患者中未检测到IgA和IgG，但10名患者中有4名（40%）表现出IgM与精子尾尖结合。在评估的4名青春期后CF男性患者中，有3名（75%）显示出高抗精子抗体（ASA）水平。7名CBAVD患者中有5名（71%）检测到ASA；发现IgG（n = 3）和IgM（n = 4）是与精子尾尖结合的主要同种型。CF基因型分析显示，两名青春期前患者具有DeltaF508/DeltaF508 CF基因型且ASA反应呈阳性，这表明存在更早或更严重的梗阻。此外，在初始筛查中发现具有?/? CF基因型的两名CBAVD患者没有ASA。与精子发生启动相关的精子抗原性改变似乎会改变抗精子抗体同种型。对更多患者进行进一步研究可能有助于更好地理解ASA反应，以及更好地理解CF基因型与免疫反应之间可能的表型/基因型关联。
