Kang S W, Park Y S, Lee J S, Hong S I, Kim S W
Graduate School of Biotechnology, Korea University, 1 Anam-dong, Sungbuk-ku, Seoul 136-701, South Korea.
Bioresour Technol. 2004 Jan;91(2):153-6. doi: 10.1016/s0960-8524(03)00172-x.
To investigate the production of cellulases and hemicellulases from Aspergillus niger KK2, solid state fermentation (SSF) was performed by using different ratios of rice straw and wheat bran. When A. niger KK2 was grown on rice straw alone as a solid support in SSF, the maximum FPase activity was 19.5 IU g(-1) in 4 days. Also, CMCase (129 IU g(-1)), beta-glucosidase (100 IU g(-1)), xylanase (5070 IU g(-1)) and beta-xylosidase (193 IU g(-1)) activities were concurrently obtained after 5-6 days of fermentation. The higher enzyme activities produced by A. niger KK2 is a significant advantage from the viewpoint of practical saccharification reaction. Cellulases and hemicellulases produced by A. niger KK2 might be applied to pulp and paper industry, feed industry and chemical industry.
为了研究黑曲霉KK2产生纤维素酶和半纤维素酶的情况，采用稻草和麦麸的不同比例进行固态发酵（SSF）。当黑曲霉KK2在固态发酵中仅以稻草作为固体支持物生长时，4天内最高的滤纸酶（FPase）活性为19.5 IU g(-1)。此外，发酵5 - 6天后同时获得了羧甲基纤维素酶（CMCase，129 IU g(-1)）、β-葡萄糖苷酶（100 IU g(-1)）、木聚糖酶（5070 IU g(-1)）和β-木糖苷酶（193 IU g(-1)）的活性。从实际糖化反应的角度来看，黑曲霉KK2产生的较高酶活性是一个显著优势。黑曲霉KK2产生的纤维素酶和半纤维素酶可能应用于制浆造纸工业、饲料工业和化学工业。
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