Agricultural Sciences Institute (ICIAG-UFU), Uberlândia Federal University, Uberlândia, Minas Gerais, 38405-320, Brazil.
Agricultural Sciences Institute (ICA-UFMG), Minas Gerais Federal University, Montes Claros, Minas Gerais, 39404-547, Brazil.
Appl Biochem Biotechnol. 2019 Sep;189(1):37-48. doi: 10.1007/s12010-019-02991-6. Epub 2019 Mar 13.
This study evaluated the production of lignocellulose-degrading enzymes by solid-state fermentation (SSF) using a microbial consortium of Aspergillus fumigatus SCBM6 and A. niger SCBM1 (AFN extract). The fungal strains were cultivated in sugarcane bagasse (SCB) and wheat bran (WB) as lignocellulosic substrates for 7 days at 30 °C. After SSF, the highest peaks of enzyme production were 150 and 80 U g for β-xylosidase and β-glucosidase at 48 h, 375 U g for xylanase at 96 h, and 80 U g for endoglucanase and 4 U g for cellulase activity on filter paper (FPase) at 144 h. The efficiency of the produced AFN extract was investigated in the enzymatic hydrolysis of crude biomass sorghum (BS) and after the removal of extractives (ES). After saccharification, the glucose and xylose concentrations were 10× superior in ES than in BS hydrolysate (2.5 g L after 12 h). The presence of inhibitors of alcoholic fermentation, such as formic acid, was also reduced in ES hydrolysates, indicating that the removal of extractives positively contributed to the effectiveness of enzymatic hydrolysis of biomass sorghum using AFN extract.
本研究评估了固态发酵（SSF）中使用烟曲霉 SCBM6 和黑曲霉 SCBM1（AFN 提取物）微生物共培养物生产木质纤维素降解酶。在 30°C 下，将真菌菌株在甘蔗渣（SCB）和小麦麸皮（WB）作为木质纤维素底物中培养 7 天。在 SSF 之后，β-木糖苷酶和β-葡萄糖苷酶在 48 小时时达到最高酶活峰值，分别为 150 和 80 U/g，木聚糖酶在 96 小时时达到 375 U/g，内切葡聚糖酶和滤纸纤维素酶（FPase）活性在 144 小时时达到 80 U/g 和 4 U/g。研究了所产生的 AFN 提取物在粗生物质高粱（BS）的酶解和提取物去除（ES）后的效率。糖化后，ES 中的葡萄糖和木糖浓度比 BS 水解液高 10 倍（12 小时后为 2.5 g/L）。ES 水解液中还减少了酒精发酵抑制剂，如甲酸，这表明去除提取物对使用 AFN 提取物对生物质高粱的酶解效果有积极贡献。
