Cheah Fook-Choe, Darlow Brian A, Winterbourn Christine C
Free Radical Research Group, Department of Pathology, Christchurch School of Medicine and Health Sciences, University of Otago, Christchurch, New Zealand.
Biol Neonate. 2003;84(4):338-41. doi: 10.1159/000073644.
We developed a bedside method for collecting exhaled breath condensate (EBC) from neonates who were ventilated or receiving nasal continuous positive airway pressure (CPAP) and analyzed their EBC for hydrogen peroxide levels. A sufficient volume for analysis could be collected over 25-40 min from neonates on the ventilator and nasal CPAP (medians 5.3 and 2.7 ml, respectively). There was no significant difference between hydrogen peroxide levels from neonates on a ventilator or CPAP (median 0.28 vs. 0.38 microM, p = 0.06) and these were no different from a background with the ventilator or CPAP system alone (median for each 0.31 microM). The dilution of breath condensate by humidified gases plus the existence of background hydrogen peroxide resulted in this collecting setup being insufficiently sensitive to use for the detection of exhaled hydrogen peroxide in infants who were ventilated or on nasal CPAP.
我们开发了一种床边方法，用于从接受机械通气或鼻持续气道正压通气（CPAP）的新生儿中收集呼出气冷凝液（EBC），并分析其EBC中的过氧化氢水平。在25-40分钟内，可以从使用呼吸机和鼻CPAP的新生儿中收集到足够用于分析的体积（中位数分别为5.3和2.7毫升）。使用呼吸机或CPAP的新生儿的过氧化氢水平之间没有显著差异（中位数分别为0.28和0.38微摩尔，p = 0.06），并且与仅使用呼吸机或CPAP系统时的背景水平没有差异（每种情况的中位数均为0.31微摩尔）。加湿气体对呼出气冷凝液的稀释以及背景过氧化氢的存在导致这种收集设置对于检测接受机械通气或鼻CPAP的婴儿呼出的过氧化氢不够灵敏。
