Van Hoydonck P G A, Wuyts W A, Vanaudenaerde B M, Schouten E G, Dupont L J, Temme E H M
Dept of Public Health, Division of Nutritional Epidemiology, Catholic University of Leuven, Leuven, Belgium.
Eur Respir J. 2004 Feb;23(2):189-92. doi: 10.1183/09031936.03.00049403.
Exhaled breath condensate (EBC) provides a noninvasive means of sampling the lower respiratory tract. Collection of EBC might be useful in the assessment of airway oxidative stress in smokers. The aim of this study was to determine 8-isoprostane and hydrogen peroxide levels in EBC, and, in addition, to investigate the reproducibility of these measurements. EBC samples were collected from 12 healthy male smokers at three time points within 1 week. 8-isoprostane and H2O2 were measured in nonconcentrated EBC using immunochemical and colorimetric assays, respectively. 8-isoprostane and H2O2 were detected in only 36 and 47% of all EBC samples, respectively. It was not possible to calculate the within-subject variation in a reliable manner since only three of the 12 smokers exhibited detectable 8-isoprostane concentrations on all three occasions (mean 4.6 pg x mL(-1); range 3.9-7.7 pg x mL(-1)), whereas H2O2 could not be detected on all three occasions in any of the smokers. Spiking experiments revealed a recovery of 83.5-109.5% for 8-isoprostane and 69.9-129.0%, for H2O2 in fresh EBC samples. It was concluded that levels of 8-isoprostane and hydrogen peroxide cannot be reproducibly assessed in exhaled breath condensate from healthy smokers because of their low concentration and/or the lack of sensitivity of the available assays.
呼出气冷凝液（EBC）提供了一种对下呼吸道进行采样的非侵入性方法。收集EBC可能有助于评估吸烟者气道的氧化应激。本研究的目的是测定EBC中8-异前列腺素和过氧化氢的水平，此外，还研究这些测量的可重复性。在1周内的三个时间点从12名健康男性吸烟者中收集EBC样本。分别使用免疫化学和比色法在未浓缩的EBC中测量8-异前列腺素和H2O2。在所有EBC样本中，分别仅在36%和47%的样本中检测到8-异前列腺素和H2O2。由于12名吸烟者中只有3人在所有三个检测时间点均表现出可检测到的8-异前列腺素浓度（平均4.6 pg x mL(-1)；范围3.9 - 7.7 pg x mL(-1)），而在任何吸烟者的所有三个检测时间点均未检测到H2O2，因此无法可靠地计算受试者内变异。加标实验显示，新鲜EBC样本中8-异前列腺素的回收率为83.5 - 109.5%，H2O2的回收率为69.9 - 129.0%。得出的结论是，由于健康吸烟者呼出气冷凝液中8-异前列腺素和过氧化氢的浓度较低和/或现有检测方法缺乏敏感性，因此无法对其水平进行可重复评估。
