慢性丙型肝炎病毒感染患者中辅助性T细胞1和辅助性T细胞2细胞因子的血清谱。

Serum profile of T helper 1 and T helper 2 cytokines in patients with chronic hepatitis C virus infection.

作者信息

Abayli Bahri, Canataroğlu Abdullah, Akkiz Hikmet

机构信息

Department of Internal Medicine, Faculty of Medicine, Cukurova University, Adana, Turkey.

出版信息

Turk J Gastroenterol. 2003 Mar;14(1):7-11.

PMID:14593531

Abstract

BACKGROUND/AIMS: T cell immunoregulatory cytokines may play a crucial role in the host response to hepatitis C virus infection. While T-helper type 1 cytokines are required for host antiviral immune responses, T-helper type 2 cytokines can inhibit the development of these effector mechanisms. The aim of the present study was to determine T-helper type 1 and T-helper type 2 cytokine levels in chronic hepatitis C infection.

METHODS

Serum levels of T-helper type 1 cytokine, interferon-gamma (IFN-g), and T-helper type 2 cytokines, IL-4 and IL-10 were measured in 30 patients with hepatitis C infection and 25 healthy controls using ELISA. Serum levels of alanine transaminase were also assessed in both patients and controls. Histologic activity score was evaluated in the chronic hepatic C infected patients.

RESULTS

Serum levels of IFN-g were 59.03+/-46.24 pg/mL, IL-4 were 213.59+/-135.67 pg/mL and IL-10 were 106.73+/-60.85 pg/mL in hepatitis C infected patients whereas in healthy controls they were found to be 61.84+/-54.87 pg/mL, 67.39+/-59.74 pg/mL and 60.14+/-50.73 pg/mL respectively. Serum levels of IL-4, IL-10 but not IFN-g were found to be significantly increased in chronic HCV patients compared to those of control subjects.

CONCLUSIONS

Our findings may suggest the involvement of Th2 cytokines in the pathogenesis of chronic hepatitis C virus liver disease.

摘要

背景/目的：T细胞免疫调节细胞因子可能在宿主对丙型肝炎病毒感染的反应中起关键作用。虽然1型辅助性T细胞细胞因子是宿主抗病毒免疫反应所必需的，但2型辅助性T细胞细胞因子可抑制这些效应机制的发展。本研究的目的是确定慢性丙型肝炎感染中1型辅助性T细胞和2型辅助性T细胞细胞因子的水平。

方法

采用酶联免疫吸附测定法（ELISA）测量30例丙型肝炎感染患者和25例健康对照者血清中1型辅助性T细胞细胞因子干扰素-γ（IFN-γ）以及2型辅助性T细胞细胞因子白细胞介素-4（IL-4）和白细胞介素-10（IL-10）的水平。还评估了患者和对照者的血清丙氨酸转氨酶水平。对慢性丙型肝炎感染患者的组织学活性评分进行了评估。

结果

丙型肝炎感染患者血清中IFN-γ水平为59.03±46.24 pg/mL，IL-4水平为213.59±135.67 pg/mL，IL-10水平为106.73±60.85 pg/mL；而在健康对照者中，这些水平分别为61.84±54.87 pg/mL、67.39±59.74 pg/mL和60.14±50.73 pg/mL。与对照受试者相比，慢性丙型肝炎患者血清中IL-4、IL-10水平显著升高，而IFN-γ水平未升高。