Hickey W J, Brenner V, Focht D D
Department of Soil and Environmental Science, University of California, Riverside.
FEMS Microbiol Lett. 1992 Nov 1;77(1-3):175-80. doi: 10.1016/0378-1097(92)90151-d.
Pseudomonas sp. strain UCR2 was isolated from a multi-chemostat mating experiment between a chlorobenzoate-degrader, Pseudomonas aeruginosa strain JB2, and a chlorobiphenyl-degrader, Arthrobacter sp. strain B1Barc. Strain UCR2 differed from either of the parental organisms in that it grew on both 2-chloro- and 2,5-dichlorobiphenyl with concomitant release of chloride. Phenotypic typing by the Biolog system indicated that strain UCR2 shared greater similarity with strain JB2 (88%) than strain B1Barc (3%). In DNA:DNA hybridization experiments, genomic DNA from strain UCR2 hybridized with both strain JB2 and strain B1Barc, with the former pairing yielding a much stronger signal than the latter. In contrast, no hybridization whatsoever was observed when the parental organisms strains JB2 and B1Barc were probed against each other.
假单胞菌属菌株UCR2是从一株氯苯甲酸降解菌铜绿假单胞菌菌株JB2和一株氯联苯降解菌节杆菌属菌株B1Barc之间的多恒化器交配实验中分离出来的。菌株UCR2与亲本菌株的不同之处在于，它能在2-氯联苯和2,5-二氯联苯上生长，并伴随氯离子的释放。Biolog系统的表型分型表明，菌株UCR2与菌株JB2的相似度（88%）高于与菌株B1Barc的相似度（3%）。在DNA:DNA杂交实验中，菌株UCR2的基因组DNA与菌株JB2和菌株B1Barc都发生了杂交，前者配对产生的信号比后者强得多。相比之下，当亲本菌株JB2和B1Barc相互检测时，未观察到任何杂交现象。
