Havel J, Reineke W
Bergische Universität-Gesamthochschule Wuppertal, Germany.
Appl Microbiol Biotechnol. 1995 Oct;43(5):914-9. doi: 10.1007/BF02431928.
The influence of different forms of substrate administration (either through the vapour phase or the liquid phase) on growth of two bacterial strains on biphenyl, 2-chlorobiphenyl, and 3,5-dichlorobiphenyl has been investigated. During growth with all three compounds, the availability of the substrate for the cells turned out to be the growth-limiting factor, even in liquid culture with excess substrate supplied to the medium. Growth on biphenyl and 2-chlorobiphenyl could be greatly enhanced if the substrate was distributed on a folded filter providing a large surface, which was placed in the vapour phase of the culture flask. This was not possible in the case of 3,5-dichlorobiphenyl. Here growth accelerated after accumulation of a yellow meta cleavage product. Through measurement of the surface tension it was shown that this yellow compound possessed detergent-like activities, increasing the amount of 3,5-dichlorobiphenyl dissolved in the medium.
研究了不同形式的底物给药（通过气相或液相）对两种细菌菌株在联苯、2-氯联苯和3,5-二氯联苯上生长的影响。在使用所有三种化合物进行生长的过程中，结果表明，即使在向培养基中供应过量底物的液体培养中，细胞可利用的底物也是生长限制因素。如果将底物分布在提供大表面积的折叠滤纸上，并将其置于培养瓶的气相中，则联苯和2-氯联苯上的生长会大大增强。对于3,5-二氯联苯则不可能如此。在此，黄色间位裂解产物积累后生长加速。通过测量表面张力表明，这种黄色化合物具有类似洗涤剂的活性，增加了溶解在培养基中的3,5-二氯联苯的量。
