• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Engineering hybrid pseudomonads capable of utilizing a wide range of aromatic hydrocarbons and of efficient degradation of trichloroethylene.构建能够利用多种芳烃并高效降解三氯乙烯的杂种假单胞菌。
J Bacteriol. 1996 Jul;178(14):4039-46. doi: 10.1128/jb.178.14.4039-4046.1996.
2
Efficient degradation of trichloroethylene by a hybrid aromatic ring dioxygenase.一种杂合芳香环双加氧酶对三氯乙烯的高效降解
J Bacteriol. 1994 Apr;176(7):2121-3. doi: 10.1128/jb.176.7.2121-2123.1994.
3
Construction of hybrid biphenyl (bph) and toluene (tod) genes for functional analysis of aromatic ring dioxygenases.构建用于芳香环双加氧酶功能分析的杂合联苯（bph）和甲苯（tod）基因。
Gene. 1994 Jan 28;138(1-2):27-33. doi: 10.1016/0378-1119(94)90779-x.
4
Gene components responsible for discrete substrate specificity in the metabolism of biphenyl (bph operon) and toluene (tod operon).负责联苯代谢（bph操纵子）和甲苯代谢（tod操纵子）中离散底物特异性的基因组件。
J Bacteriol. 1993 Aug;175(16):5224-32. doi: 10.1128/jb.175.16.5224-5232.1993.
5
Directed evolution of biphenyl dioxygenase: emergence of enhanced degradation capacity for benzene, toluene, and alkylbenzenes.联苯双加氧酶的定向进化：对苯、甲苯和烷基苯的降解能力增强的出现
J Bacteriol. 2001 Sep;183(18):5441-4. doi: 10.1128/JB.183.18.5441-5444.2001.
6
Functional analyses of Bph-Tod hybrid dioxygenase, which exhibits high degradation activity toward trichloroethylene.对三氯乙烯具有高降解活性的Bph-Tod杂合双加氧酶的功能分析。
J Biol Chem. 2001 Aug 10;276(32):29833-8. doi: 10.1074/jbc.M102025200. Epub 2001 Jun 4.
7
Addition of aromatic substrates restores trichloroethylene degradation activity in Pseudomonas putida F1.添加芳香族底物可恢复恶臭假单胞菌F1中的三氯乙烯降解活性。
Appl Environ Microbiol. 2004 May;70(5):2830-5. doi: 10.1128/AEM.70.5.2830-2835.2004.
8
Trichloroethylene degradation by Escherichia coli containing the cloned Pseudomonas putida F1 toluene dioxygenase genes.含有克隆的恶臭假单胞菌F1甲苯双加氧酶基因的大肠杆菌对三氯乙烯的降解作用
Appl Environ Microbiol. 1989 Dec;55(12):3162-6. doi: 10.1128/aem.55.12.3162-3166.1989.
9
[Molecular biological characterization of biphenyl-degrading bacteria and identification of the biphenyl 2,3-dioxygenase α-subunit genes].
Mikrobiologiia. 2014 Jan-Feb;83(1):63-71.
10
Degradation of chlorobiphenyls catalyzed by the bph-encoded biphenyl-2,3-dioxygenase and biphenyl-2,3-dihydrodiol-2,3-dehydrogenase of Pseudomonas sp. LB400.由假单胞菌属LB400菌株的bph编码的联苯-2,3-双加氧酶和联苯-2,3-二氢二醇-2,3-脱氢酶催化的氯代联苯降解作用。
FEMS Microbiol Lett. 1995 Nov 15;133(3):259-64. doi: 10.1111/j.1574-6968.1995.tb07894.x.

引用本文的文献

1
Microbiota associated with the migration and transformation of chlorinated aliphatic hydrocarbons in groundwater.与地下水氯化脂肪烃的迁移和转化相关的微生物群落。
Environ Geochem Health. 2013 Aug;35(4):535-49. doi: 10.1007/s10653-013-9513-3. Epub 2013 Feb 19.
2
Microbial degradation of petroleum hydrocarbon contaminants: an overview.石油烃污染物的微生物降解：综述
Biotechnol Res Int. 2011;2011:941810. doi: 10.4061/2011/941810. Epub 2010 Sep 13.
3
Improving dioxygenase stability by gene chromosome insertion: implementation in immobilized-cell systems.通过基因染色体插入提高双加氧酶稳定性：在固定化细胞系统中的应用
Curr Microbiol. 2004 Dec;49(6):390-5. doi: 10.1007/s00284-004-4283-x.
4
Complete sequence of a 184-kilobase catabolic plasmid from Sphingomonas aromaticivorans F199.来自食芳鞘氨醇单胞菌F199的184千碱基分解代谢质粒的完整序列。
J Bacteriol. 1999 Mar;181(5):1585-602. doi: 10.1128/JB.181.5.1585-1602.1999.
5
Identification of chlorobenzene dioxygenase sequence elements involved in dechlorination of 1,2,4,5-tetrachlorobenzene.鉴定参与1,2,4,5-四氯苯脱氯反应的氯苯双加氧酶序列元件。
J Bacteriol. 1998 Nov;180(21):5520-8. doi: 10.1128/JB.180.21.5520-5528.1998.
6
Construction and use of an ipb DNA module to generate Pseudomonas strains with constitutive trichloroethene and isopropylbenzene oxidation activity.构建并使用一个ipb DNA模块来生成具有组成型三氯乙烯和异丙苯氧化活性的假单胞菌菌株。
Appl Environ Microbiol. 1998 Jul;64(7):2454-62. doi: 10.1128/AEM.64.7.2454-2462.1998.
7
Trichloroethylene biodegradation by mesophilic and psychrophilic ammonia oxidizers and methanotrophs in groundwater microcosms.中温及嗜冷氨氧化菌和甲烷氧化菌在地下水微宇宙中对三氯乙烯的生物降解作用
Appl Environ Microbiol. 1997 Oct;63(10):3866-71. doi: 10.1128/aem.63.10.3866-3871.1997.
8
Glutathione S-transferase-encoding gene as a potential probe for environmental bacterial isolates capable of degrading polycyclic aromatic hydrocarbons.谷胱甘肽S-转移酶编码基因作为能够降解多环芳烃的环境细菌分离株的潜在探针。
Appl Environ Microbiol. 1997 Aug;63(8):3286-90. doi: 10.1128/aem.63.8.3286-3290.1997.
9
Functional analyses of a variety of chimeric dioxygenases constructed from two biphenyl dioxygenases that are similar structurally but different functionally.对由两种结构相似但功能不同的联苯双加氧酶构建的多种嵌合双加氧酶进行功能分析。
J Bacteriol. 1997 Jun;179(12):3936-43. doi: 10.1128/jb.179.12.3936-3943.1997.

本文引用的文献

1
Trichloroethylene biodegradation by a methane-oxidizing bacterium.甲烷氧化菌对三氯乙烯的生物降解。
Appl Environ Microbiol. 1988 Apr;54(4):951-6. doi: 10.1128/aem.54.4.951-956.1988.
2
Gene components responsible for discrete substrate specificity in the metabolism of biphenyl (bph operon) and toluene (tod operon).负责联苯代谢（bph操纵子）和甲苯代谢（tod操纵子）中离散底物特异性的基因组件。
J Bacteriol. 1993 Aug;175(16):5224-32. doi: 10.1128/jb.175.16.5224-5232.1993.
3
Genetic analysis of a Pseudomonas locus encoding a pathway for biphenyl/polychlorinated biphenyl degradation.对编码联苯/多氯联苯降解途径的假单胞菌基因座的遗传分析。
Gene. 1993 Aug 16;130(1):47-55. doi: 10.1016/0378-1119(93)90345-4.
4
Metabolism of polyhalogenated compounds by a genetically engineered bacterium.一种基因工程菌对多卤代化合物的代谢
Nature. 1994 Apr 14;368(6472):627-9. doi: 10.1038/368627a0.
5
Efficient degradation of trichloroethylene by a hybrid aromatic ring dioxygenase.一种杂合芳香环双加氧酶对三氯乙烯的高效降解
J Bacteriol. 1994 Apr;176(7):2121-3. doi: 10.1128/jb.176.7.2121-2123.1994.
6
Trichloroethylene and chloroform degradation by a recombinant pseudomonad expressing soluble methane monooxygenase from Methylosinus trichosporium OB3b.通过表达来自甲基孢囊菌OB3b的可溶性甲烷单加氧酶的重组假单胞菌降解三氯乙烯和氯仿
Appl Environ Microbiol. 1994 Jul;60(7):2473-82. doi: 10.1128/aem.60.7.2473-2482.1994.
7
The biphenyl/polychlorinated biphenyl-degradation locus (bph) of Pseudomonas sp. LB400 encodes four additional metabolic enzymes.假单胞菌属LB400菌株的联苯/多氯联苯降解基因座（bph）编码另外四种代谢酶。
Gene. 1994 Jun 24;144(1):9-16. doi: 10.1016/0378-1119(94)90196-1.
8
Nucleotide sequence and functional analysis of the meta-cleavage pathway involved in biphenyl and polychlorinated biphenyl degradation in Pseudomonas sp. strain KKS102.假单胞菌属菌株KKS102中参与联苯和多氯联苯降解的间位裂解途径的核苷酸序列及功能分析
J Bacteriol. 1994 Jul;176(14):4269-76. doi: 10.1128/jb.176.14.4269-4276.1994.
9
Designing microorganisms for the treatment of toxic wastes.设计用于处理有毒废物的微生物。
Annu Rev Microbiol. 1994;48:525-57. doi: 10.1146/annurev.mi.48.100194.002521.
10
Characterization of biphenyl catabolic genes of gram-positive polychlorinated biphenyl degrader Rhodococcus sp. strain RHA1.革兰氏阳性多氯联苯降解菌红球菌属菌株RHA1中多氯联苯分解代谢基因的特性分析
Appl Environ Microbiol. 1995 Jun;61(6):2079-85. doi: 10.1128/aem.61.6.2079-2085.1995.

构建能够利用多种芳烃并高效降解三氯乙烯的杂种假单胞菌。

Engineering hybrid pseudomonads capable of utilizing a wide range of aromatic hydrocarbons and of efficient degradation of trichloroethylene.

作者信息

Suyama A, Iwakiri R, Kimura N, Nishi A, Nakamura K, Furukawa K

机构信息

Department of Agricultural Chemistry, Kyushu University, Japan.

出版信息

J Bacteriol. 1996 Jul;178(14):4039-46. doi: 10.1128/jb.178.14.4039-4046.1996.

DOI:10.1128/jb.178.14.4039-4046.1996
PMID:8763929
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC178158/
Abstract

We constructed hybrid Pseudomonas strains in which the bphA1 gene (coding for a large subunit of biphenyl dioxygenase) is replaced with the todC1 gene (coding for a large subunit of toluene dioxygenase of Pseudomonas putida Fl) within chromosomal biphenyl-catabolic bph gene clusters. Such hybrid strains gained the novel capability to grow on a wide range of aromatic hydrocarbons, and, more interestingly, they degraded chloroethenes such as trichloroethylene and cis-1,2-dichloroethylene very efficiently.

摘要

我们构建了杂种假单胞菌菌株，其中在染色体联苯分解代谢bph基因簇内，bphA1基因（编码联苯双加氧酶的大亚基）被todC1基因（编码恶臭假单胞菌Fl甲苯双加氧酶的大亚基）所取代。这类杂种菌株获得了在多种芳香烃上生长的新能力，更有趣的是，它们能非常有效地降解氯乙烯，如三氯乙烯和顺式-1,2-二氯乙烯。