构建能够利用多种芳烃并高效降解三氯乙烯的杂种假单胞菌。
Engineering hybrid pseudomonads capable of utilizing a wide range of aromatic hydrocarbons and of efficient degradation of trichloroethylene.
作者信息
Suyama A, Iwakiri R, Kimura N, Nishi A, Nakamura K, Furukawa K
机构信息
Department of Agricultural Chemistry, Kyushu University, Japan.
出版信息
J Bacteriol. 1996 Jul;178(14):4039-46. doi: 10.1128/jb.178.14.4039-4046.1996.
Abstract
We constructed hybrid Pseudomonas strains in which the bphA1 gene (coding for a large subunit of biphenyl dioxygenase) is replaced with the todC1 gene (coding for a large subunit of toluene dioxygenase of Pseudomonas putida Fl) within chromosomal biphenyl-catabolic bph gene clusters. Such hybrid strains gained the novel capability to grow on a wide range of aromatic hydrocarbons, and, more interestingly, they degraded chloroethenes such as trichloroethylene and cis-1,2-dichloroethylene very efficiently.
摘要
我们构建了杂种假单胞菌菌株，其中在染色体联苯分解代谢bph基因簇内，bphA1基因（编码联苯双加氧酶的大亚基）被todC1基因（编码恶臭假单胞菌Fl甲苯双加氧酶的大亚基）所取代。这类杂种菌株获得了在多种芳香烃上生长的新能力，更有趣的是，它们能非常有效地降解氯乙烯，如三氯乙烯和顺式-1,2-二氯乙烯。
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