利用二价铕和聚氨基羧酸盐配体瞬间化学计量地生成蛋白质活性位点的强还原态。

Instantaneous, stoichiometric generation of powerfully reducing states of protein active sites using Eu(II) and polyaminocarboxylate ligands.

作者信息

Vincent Kylie A, Tilley Gareth J, Quammie Nina C, Streeter Ian, Burgess Barbara K, Cheesman Myles R, Armstrong Fraser A

机构信息

Inorganic Chemistry Laboratory, Oxford University, South Parks Road, Oxford, UK OX1 3QR.

出版信息

Chem Commun (Camb). 2003 Oct 21(20):2590-1. doi: 10.1039/b308188e.

DOI:10.1039/b308188e

PMID:14594295

Abstract

Addition of an equivalent of a polyaminocarboxylate ligand (L) to a solution of a redox protein and the aqua Eu2+ ion results in the instantaneous in situ generation of a very powerful reductant Eu(II)-L that can rapidly drive an electron stoichiometrically onto a redox centre having an extremely negative reduction potential (lower than -1 V): this is exemplified by straightforward generation of the super-reduced state of the Fe-protein of nitrogenase.

摘要

向氧化还原蛋白和水合 Eu2+ 离子的溶液中加入等当量的聚氨基羧酸盐配体（L），会立即原位生成一种非常强大的还原剂 Eu(II)-L，它可以将电子按化学计量迅速驱动到具有极低还原电位（低于 -1 V）的氧化还原中心上：这可以通过直接生成固氮酶铁蛋白的超还原态来例证。