Department of Nephrology and Rheumatology, Molecular and Optical Live Cell Imaging, Center for Internal Medicine, University Medicine Goettingen, Göttingen, Germany.
Cytometry A. 2010 Jul;77(7):667-76. doi: 10.1002/cyto.a.20931.
This review focuses on technical advances in fluorescence microscopy techniques including laser scanning techniques, fluorescence-resonance energy transfer (FRET) microscopy, fluorescence lifetime imaging (FLIM), stimulated emission depletion (STED)-based super-resolution microscopy, scanning confocal endomicroscopes, thin-sheet laser imaging microscopy (TSLIM), and tomographic techniques such as early photon tomography (EPT) as well as on clinical laser-based endoscopic and microscopic techniques. We will also discuss the new developments in the field of fluorescent dyes and fluorescent genetic reporters that enable new possibilities in high-resolution and molecular imaging both in in vitro and in vivo. Small animal and tissue imaging benefit from the development of new fluorescent proteins, dyes, and sensing constructs that operate in the far red and near-infrared spectrum.
本文综述了荧光显微镜技术的技术进展，包括激光扫描技术、荧光共振能量转移（FRET）显微镜、荧光寿命成像（FLIM）、基于受激发射损耗（STED）的超分辨率显微镜、扫描共聚焦内窥显微镜、薄切片激光成像显微镜（TSLIM）以及体视学技术，如早期光子断层扫描（EPT），以及临床基于激光的内镜和显微镜技术。我们还将讨论荧光染料和荧光遗传报告基因领域的新进展，这些进展使在体外和体内进行高分辨率和分子成像成为可能。新型荧光蛋白、染料和传感构建体的发展使小动物和组织成像受益，这些构建体在远红和近红外光谱范围内工作。
