CSIRO Materials Science and Engineering, PO Box 56, Highett, Melbourne, VIC 3190, Australia.
Lab Chip. 2010 Jun 7;10(11):1355-64. doi: 10.1039/b924271f. Epub 2010 Mar 16.
This review focuses on the use of Förster Resonance Energy Transfer (FRET) to monitor intra- and intermolecular reactions occurring in microfluidic reactors. Microfluidic devices have recently been used for performing highly efficient and miniaturised biological assays for the analysis of biological entities such as cells, proteins and nucleic acids. Microfluidic assays are characterised by nanolitre to femtolitre reaction volumes, which necessitates the adoption of a sensitive optical detection scheme. FRET serves as a strong 'spectroscopic ruler' for elucidating the tertiary structure of biomolecules, as the efficiency of the non-radiative energy transfer is extremely sensitive to nanoscale changes in the separation between donor and acceptor markers attached to the biomolecule of interest. In this review, we will review the implementation of various microfluidic assays which employ FRET for diverse applications in the biomedical field, along with the advantages and disadvantages of the various approaches. The future prospects for development of microfluidic devices incorporating FRET detection will be discussed.
这篇综述专注于使用Förster 共振能量转移（FRET）来监测在微流反应器中发生的分子内和分子间反应。微流控器件最近已被用于进行高效和微型化的生物分析，用于分析细胞、蛋白质和核酸等生物实体。微流控分析的特点是纳升到飞升的反应体积，这需要采用灵敏的光学检测方案。FRET 可作为阐明生物分子三级结构的有力“光谱标尺”，因为供体和受体标记物与感兴趣的生物分子之间的分离的纳米级变化对非辐射能量转移的效率非常敏感。在这篇综述中，我们将回顾各种微流控分析的实施情况，这些分析采用 FRET 进行生物医学领域的各种应用，并讨论各种方法的优缺点。还将讨论结合 FRET 检测的微流控器件的未来发展前景。
