杜氏利什曼原虫或硕大利什曼原虫感染的人外周血单个核细胞中肿瘤坏死因子α和白细胞介素-18的差异诱导
Differential induction of TNFalpha and IL-18 in human peripheral blood mononuclear cells infected with Leishmania major or Leishmania donovani.
作者信息
Iannello D, Arena A, Buemi C, Calapai M, Stassi G, Gazzara D, Mastroeni P
机构信息
Department of Surgical Sciences, Unit of Microbiology, Policlinico Universitario, University of Messina, Via C. Valeria, 98125 Messina, Italy.
出版信息
New Microbiol. 2003 Oct;26(4):399-404.
Several cytokines are involved in the host response to Leishmania. However, the role played by cytokines during infection with different species of Leishmania is not univocal. In this work, the production of tumor necrosis factor alpha (TNFalpha) and interleukin 18 (IL-18) during interaction of human phagocytes with Leishmania major or L. donovani was comparatively investigated. Peripheral blood mononuclear cells (PBMC) and monocytes from healthy donors were used. The release of TNFalpha and IL-18 during infection of cells with different species of Leishmania "in vitro" was evaluated. L. donovani induced in both PBMC and monocytes significantly more TNFalpha and IL-18 with respect to L. major. The amounts of TNFalpha released by PBMC were always significantly higher than those released by monocytes of the same donors.
几种细胞因子参与宿主对利什曼原虫的反应。然而，细胞因子在感染不同种类利什曼原虫过程中所起的作用并非单一。在这项研究中，对人类吞噬细胞与硕大利什曼原虫或杜氏利什曼原虫相互作用期间肿瘤坏死因子α（TNFα）和白细胞介素18（IL-18）的产生进行了比较研究。使用了来自健康供体的外周血单核细胞（PBMC）和单核细胞。评估了不同种类利什曼原虫“体外”感染细胞期间TNFα和IL-18的释放。相对于硕大利什曼原虫，杜氏利什曼原虫在PBMC和单核细胞中均诱导产生显著更多的TNFα和IL-18。PBMC释放的TNFα量始终显著高于相同供体单核细胞释放的量。
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