Numazaki Kei, Umetsu Masao, Adachi Noriaki
Department of Pediatrics, Sapporo Medical University School of Medicine, S.1 W.16 Chuo-ku, Sapporo, 060-8543, Japan.
In Vivo. 2003 Sep-Oct;17(5):421-4.
To investigate the etiology of Mycoplasma pneumoniae infections we conducted a prospective study covering the total pediatric population in Hokkaido, Japan. Paired sera for serologic assays were available for more than half of the cases (n = 921; 398 as pneumonia and 523 as bronchitis). The nasopharyngeal swabs were also collected for isolation and PCR study. The types of P1 gene from clinical isolates of M. pneumoniae obtained from two different areas of Hokkaido, Sapporo and Kushiro, were determined by PCR-RFLP assay. M. pneumoniae was identified in 174 (43.7%) out of 398 patients with pneumonia and was identified in 78 (14.9%) out of 523 (86.2%) patients with bronchitis. P1 genes of 14 clinical isolates of M. pneumoniae were classified into 13 group II and 1 group I. Two clinical isolates were unclassified by PCR-RFLP assay. Mycoplasma infections were seen even in patients less than 5 years of age. Generation of antigenic variation by DNA recombination may occur in clinical isolates.
为了研究肺炎支原体感染的病因，我们开展了一项前瞻性研究，涵盖了日本北海道的所有儿科患者。超过半数的病例（n = 921；398例为肺炎，523例为支气管炎）可获得用于血清学检测的配对血清。还采集了鼻咽拭子用于分离和PCR研究。通过PCR-RFLP分析确定了从北海道两个不同地区札幌和钏路获得的肺炎支原体临床分离株的P1基因类型。398例肺炎患者中有174例（43.7%）鉴定出肺炎支原体，523例（86.2%）支气管炎患者中有78例（14.9%）鉴定出肺炎支原体。14株肺炎支原体临床分离株的P1基因分为13个II组和1个I组。2株临床分离株通过PCR-RFLP分析无法分类。甚至在5岁以下的患者中也发现了支原体感染。临床分离株中可能发生通过DNA重组产生的抗原变异。
