Kenri Tsuyoshi, Okazaki Norio, Yamazaki Tsutomu, Narita Mitsuo, Izumikawa Kinich, Matsuoka Mayumi, Suzuki Satowa, Horino Atsuko, Sasaki Tsuguo
Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208-0011, Japan.
Kanagawa Prefectural Institute of Public Health, Chigasaki, Kanagawa 253-0087, Japan.
J Med Microbiol. 2008 Apr;57(Pt 4):469-475. doi: 10.1099/jmm.0.47634-0.
Mycoplasma pneumoniae clinical isolates obtained between 1995 and 2005 were examined to determine the prevalent genotype. One hundred and twenty-seven strains isolated from bronchitis and pneumonia patients were genotyped by a PCR-RFLP method based on nucleotide sequence polymorphisms of the p1 gene, which encodes the major adhesin protein. The typing results established that 66 of the isolates were group I strains, 45 were group II strains and 16 were group II variants. Analysis of the annual occurrence of these isolates showed a predominance of group II strains between 1995 and 2001 (n=37). No group I strain was found during this period. However, group I strains appeared in the isolates from 2002 (2/5 isolates, 40 %) and increased in specimens taken after 2003, thereby constituting a large proportion of the isolates. In 2004 and 2005, no group II strains were found among the isolates (n=49), although there were nine group II variants. Throat swabs and sputum samples obtained from patients with respiratory infections between 1997 and 2005 were also analysed by PCR-RFLP or a new nested PCR to detect the p1 gene DNA. Typing analysis of these p1 gene DNAs also showed that the group I p1 gene was not present in specimens taken before 2000, but was present and dominant in specimens taken after 2001. These results indicate that, in Japan, the prevalent type of M. pneumoniae changed from a group II strain to a group I strain around 2002.
对1995年至2005年间获得的肺炎支原体临床分离株进行检测，以确定流行基因型。采用基于编码主要黏附蛋白的p1基因核苷酸序列多态性的PCR-RFLP方法，对从支气管炎和肺炎患者中分离出的127株菌株进行基因分型。分型结果显示，66株分离株为I组菌株，45株为II组菌株，16株为II组变异株。对这些分离株年度出现情况的分析表明，1995年至2001年期间II组菌株占优势（n=37）。在此期间未发现I组菌株。然而，I组菌株在2002年的分离株中出现（2/5株，40%），并在2003年后采集的标本中增加，从而在分离株中占很大比例。2004年和2005年，在分离株（n=49）中未发现II组菌株，尽管有9株II组变异株。还通过PCR-RFLP或一种新的巢式PCR对1997年至2005年间从呼吸道感染患者中获得的咽拭子和痰液样本进行分析，以检测p1基因DNA。对这些p1基因DNA的分型分析也表明，I组p1基因在2000年前采集的标本中不存在，但在2001年后采集的标本中存在且占主导地位。这些结果表明在日本，肺炎支原体的流行类型在2002年左右从II组菌株转变为I组菌株。
