Spitsyn M A, Shershov V E, Kuznetsova V E, Barsky V E, Egorov E E, Emelyanova M A, Kreindlin E Ya, Lysov Yu P, Guseinov T O, Fesenko D E, Lapa S A, Surzhikov S A, Abramov I S, Nasedkina T V, Zasedatelev A S, Chudinov A V
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
Biochip-EIMB, Moscow, 117312, Russia.
Mol Biol (Mosk). 2015 Sep-Oct;49(5):760-9. doi: 10.7868/S002689841505016X.
To expand the informational capabilities of molecular genetic research, on the biological microchips, new indotricarbocyanine dyes that fluoresce in the near infrared (IR) spectral region have been synthesized. The developed IR dyes were studied using a biochip-based test system for detection of mutations in the BRCA1/BRCA2 and CHECK2 genes associated with breast cancer. The fluorescent label was introduced to the analyzed DNA during PCR using primers labeled with the synthesized IR dyes. An analyzer that allows recording and processing of images of fluorescent microarrays in the IR spectral region was designed and manufactured. It has been shown that the use of the synthesized dyes enables to conduct analysis in the IR region and improve the reliability of medical diagnostic tests due to low fluorescence intensity of sample components as well as of a biochip substrate and the reagents used for analysis.
为了扩展分子遗传学研究的信息能力，已在生物微芯片上合成了在近红外（IR）光谱区域发出荧光的新型吲哚三碳菁染料。使用基于生物芯片的测试系统对所开发的红外染料进行了研究，以检测与乳腺癌相关的BRCA1/BRCA2和CHECK2基因中的突变。在PCR过程中，使用合成的红外染料标记的引物将荧光标记引入到分析的DNA中。设计并制造了一种能够记录和处理红外光谱区域荧光微阵列图像的分析仪。结果表明，由于样品成分、生物芯片底物以及用于分析的试剂的低荧光强度，使用合成染料能够在红外区域进行分析并提高医学诊断测试的可靠性。
