Aign Verena, Hoheisel Jörg D
Division of Functional Genome Analysis, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, D-69120, Heidelberg, Germany.
Fungal Genet Biol. 2003 Dec;40(3):225-33. doi: 10.1016/s1087-1845(03)00106-3.
Nutrient-dependent variations in transcript levels of the filamentous fungus Neurospora crassa were studied on a microarray containing some 4700 cDNAs. Cells were grown in minimal and acetate medium. The isolated RNA was analyzed in comparison to the results obtained upon the hybridization of samples prepared from the RNA of cells grown in full medium. Altogether, 160 cDNA clones exhibited significant variations, falling into five distinct subgroups of very similar transcription profiles. This is indicative of the occurrence of a high degree of co-regulation of genes in N. crassa. Especially the regulation of the expression of proteins involved in metabolic pathways was found to be strongly regulated at the RNA level.
利用含有约4700个cDNA的微阵列，研究了丝状真菌粗糙脉孢菌转录本水平上营养依赖型变化。细胞在基本培养基和乙酸盐培养基中生长。将分离得到的RNA进行分析，并与从完全培养基中生长的细胞RNA制备的样品杂交结果进行比较。总共160个cDNA克隆表现出显著差异，分为五个转录谱非常相似的不同亚组。这表明粗糙脉孢菌中存在高度的基因共调控。特别是发现参与代谢途径的蛋白质表达在RNA水平上受到强烈调控。
