Su Bo, Liu Li, Wang Fusheng, Gui Xien, Zhao Min, Tien Po, Zhang Linqi, Chen Zhiwei
Modern Virology Research Center, College of Life Sciences, Wuhan University, Hubei 430072, PR China.
AIDS. 2003 Nov 21;17(17):2515-20. doi: 10.1097/00002030-200311210-00015.
To investigate the genetic background of HIV-1 strains among infected paid blood donors (PBD) in the Henan and Hubei provinces of China.
The polymerase chain reaction was used to amplify HIV-1 gag p17 fragments and some 5'-half or full-length viral genomes from peripheral blood mononuclear cells. The HIV-1 sequences obtained were analysed using phylogenetic and recombinant approaches.
Among the total of 62 samples studied, 59 (95.2%) came from PBD. Of those 59 PBD, 45 were collected from 14 different geographical locations in Henan, whereas the remaining 14 were from five locations in Hubei. A total of 62 HIV-1 gag p17 fragments, three 5'-half and one full HIV-1 genome sequences were obtained. Phylogenetic analysis of these sequences suggests that PBD from Henan and Hubei, despite being geographically distant, harbored a group of genetically closely related HIV-1 B' strains. No recombinant forms have been detected in this cohort.
Our data indicate that the AIDS epidemic among PBD in Henan and Hubei is caused largely by HIV-1 subtype B', which contrasts greatly with the CB' recombinant strains CRF-07 and CRF-08 currently dominating among the drug abusers in other regions of China. Our report has revealed the first complete genome background of the most dominant circulating HIV-1 strain in Henan and Hubei, which is essential for the design and development of an effective AIDS vaccine for the region.
调查中国河南和湖北有偿献血者（PBD）中HIV-1毒株的基因背景。
采用聚合酶链反应从外周血单个核细胞中扩增HIV-1 gag p17片段及部分5'-半长或全长病毒基因组。对获得的HIV-1序列进行系统发育和重组分析。
在所研究的62个样本中，59个（95.2%）来自有偿献血者。在这59名有偿献血者中，45名来自河南14个不同地理位置，其余14名来自湖北5个地点。共获得62条HIV-1 gag p17片段、3条5'-半长和1条全长HIV-1基因组序列。这些序列的系统发育分析表明，河南和湖北的有偿献血者，尽管地理位置相距遥远，但携带一组基因密切相关的HIV-1 B'毒株。该队列中未检测到重组形式。
我们的数据表明，河南和湖北有偿献血者中的艾滋病流行主要由HIV-1 B'亚型引起，这与目前在中国其他地区吸毒者中占主导地位的CB'重组毒株CRF-07和CRF-08形成了鲜明对比。我们的报告揭示了河南和湖北最主要流行的HIV-1毒株的首个完整基因组背景，这对于该地区有效艾滋病疫苗的设计和开发至关重要。
