Huang Zhiwei, McWilliams Annette, Lui Harvey, McLean David I, Lam Stephen, Zeng Haishan
Photomedicine and Optics Research Laboratory, Cancer Imaging Department, British Columbia Cancer Research Centre, 601 West 10th Avenue, Vancouver, B.C. V5Z 1L3, Canada.
Int J Cancer. 2003 Dec 20;107(6):1047-52. doi: 10.1002/ijc.11500.
Raman spectroscopy is a vibrational spectroscopic technique that can be used to optically probe the molecular changes associated with diseased tissues. The objective of our study was to explore near-infrared (NIR) Raman spectroscopy for distinguishing tumor from normal bronchial tissue. Bronchial tissue specimens (12 normal, 10 squamous cell carcinoma (SCC) and 6 adenocarcinoma) were obtained from 10 patients with known or suspected malignancies of the lung. A rapid-acquisition dispersive-type NIR Raman spectroscopy system was used for tissue Raman studies at 785 nm excitation. High-quality Raman spectra in the 700-1,800 cm(-1) range from human bronchial tissues in vitro could be obtained within 5 sec. Raman spectra differed significantly between normal and malignant tumor tissue, with tumors showing higher percentage signals for nucleic acid, tryptophan and phenylalanine and lower percentage signals for phospholipids, proline and valine, compared to normal tissue. Raman spectral shape differences between normal and tumor tissue were also observed particularly in the spectral ranges of 1,000-1,100, 1,200-1,400 and 1,500-1,700 cm(-1), which contain signals related to protein and lipid conformations and nucleic acid's CH stretching modes. The ratio of Raman intensities at 1,445 to 1,655 cm(-1) provided good differentiation between normal and malignant bronchial tissue (p < 0.0001). The results of this exploratory study indicate that NIR Raman spectroscopy provides significant potential for the noninvasive diagnosis of lung cancers in vivo based on the optic evaluation of biomolecules.
拉曼光谱是一种振动光谱技术，可用于光学探测与病变组织相关的分子变化。我们研究的目的是探索近红外（NIR）拉曼光谱用于区分肿瘤与正常支气管组织。从10例已知或疑似肺部恶性肿瘤患者获取支气管组织标本（12例正常、10例鳞状细胞癌（SCC）和6例腺癌）。使用快速采集色散型近红外拉曼光谱系统在785nm激发下进行组织拉曼研究。体外人支气管组织在700 - 1800cm⁻¹范围内的高质量拉曼光谱可在5秒内获得。正常组织与恶性肿瘤组织的拉曼光谱存在显著差异，与正常组织相比，肿瘤组织中核酸、色氨酸和苯丙氨酸的信号百分比更高，而磷脂、脯氨酸和缬氨酸的信号百分比更低。在1000 - 1100、1200 - 1400和1500 - 1700cm⁻¹光谱范围内也观察到正常组织与肿瘤组织拉曼光谱形状的差异，这些范围包含与蛋白质和脂质构象以及核酸的CH伸缩模式相关的信号。1445与1655cm⁻¹处拉曼强度的比值能很好地区分正常与恶性支气管组织（p < 0.0001）。这项探索性研究的结果表明，基于生物分子的光学评估，近红外拉曼光谱在体内肺癌的无创诊断方面具有巨大潜力。
