Karen Petr, Jirkovská Marie, Tomori Zoltán, Demjénová Erna, Janácek Jirí, Kubínová Lucie
Institute of Physiology, Academy of Sciences of the Czech Republic, Prague 4, Czech Republic.
Microsc Res Tech. 2003 Dec 1;62(5):415-22. doi: 10.1002/jemt.10405.
Computer-based visualization of large tissue volumes with high resolution based on composing series of high-resolution confocal images is presented. GlueMRC and LinkMRC programs are introduced, implementing composition of overlapping series of optical sections captured by a confocal microscope, registration and subsequent composition of successive confocal stacks. Both programs are using an interactive approach in combination with automatic algorithms for image registration. Further, the method for obtaining surface renderings of microscopical structure under study is described. For this purpose, structure contours visible in the sections are interactively digitized using a Colon plug-in module running in Ellipse environment. Then the coordinates of the contours are processed by special modules in the graphic programming environment IRIS Explorer and the structure surface is rendered. The method is shown on the 3-D reconstruction of the capillary bed of human placental villi and chick embryonic gut and its vascular bed.
本文介绍了基于高分辨率共聚焦图像序列合成的大组织体积的计算机可视化方法。文中引入了GlueMRC和LinkMRC程序，用于实现共聚焦显微镜捕获的重叠光学切片序列的合成、配准以及后续连续共聚焦堆栈的合成。这两个程序都采用了交互式方法，并结合自动算法进行图像配准。此外，还描述了获取所研究微观结构表面渲染图的方法。为此，使用在Ellipse环境中运行的Colon插件模块，对切片中可见的结构轮廓进行交互式数字化处理。然后，在图形编程环境IRIS Explorer中，通过特殊模块处理轮廓的坐标，并渲染结构表面。该方法通过对人胎盘绒毛和鸡胚肠道及其血管床的毛细血管床进行三维重建得到了展示。
