Kemp Gabré, Bergmann Carl W, Clay Ron, Van der Westhuizen Amie J, Pretorius Zacharias A
Department of Plant Sciences, University of the Free State, PO Box 339, Bloemfontein 9300, South Africa.
Mol Plant Microbe Interact. 2003 Nov;16(11):955-61. doi: 10.1094/MPMI.2003.16.11.955.
Evidence for the presence of a polygalacturonase-inhibiting protein (PGIP) from a monocotyledonous cereal is presented. A 40.3-kDa PGIP that was closely associated with the cell wall was acetone-extracted and purified from wheat (Triticum aestivum L.) leaves and stems. Wheat PGIP exhibited a highly selective inhibitory activity against endopolygalacturonase (EPG) from various fungi. Of nine EPG tested, wheat PGIP only inhibited EPG from Cochliobolus sativus, a pathogen of the tribe Poaceae. A short N-terminal amino acid sequence of wheat PGIP shows no similarity to any other characterized PGIP.
本文提供了来自单子叶谷物的多聚半乳糖醛酸酶抑制蛋白（PGIP）存在的证据。从小麦（Triticum aestivum L.）叶片和茎中丙酮提取并纯化出一种与细胞壁紧密相关的40.3 kDa的PGIP。小麦PGIP对来自各种真菌的内切多聚半乳糖醛酸酶（EPG）表现出高度选择性抑制活性。在所测试的9种EPG中，小麦PGIP仅抑制禾本科病原菌小麦根腐病菌（Cochliobolus sativus）的EPG。小麦PGIP的短N端氨基酸序列与任何其他已鉴定的PGIP均无相似性。
