Hironaka Takashi, Matsuoka Takanori, Kikuchi Ken-ichiro, Aiyama Shigeo
Department of Histology, The Nippon Dental University School of Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-8159, Japan.
Okajimas Folia Anat Jpn. 2003 Aug;80(2-3):41-6. doi: 10.2535/ofaj.80.41.
Morphological changes in the mouse sublingual gland parenchyma subjected to parasympathetic nerve block were investigated. Mice were subjected to unilateral resection of the chorda tympani, near its point of joining with the lingual nerve. After 1, 2, 3, 5, 10 or 20 weeks, the mice were killed and their sublingual glands were removed and processed for light and electron microscopy. Two weeks after resection, the space between the adjoining lobules of the glands on the treated side began to be expanded, and by 10 weeks were 10 times the size of the spaces in the glands of the untreated mice. Three weeks after resection, the lobule area decreased to about 72% of the area of glands in the untreated mice and the acinus area to about 52%. However, no significant difference was seen between the numbers of acini in each group. Electron microscopy showed that the glands on the treated side contained fewer secretory granules than the glands in the untreated mice, though there was no difference in size. Neither the lobules of the glands on the treated side nor those of the glands of the untreated mice contained many TUNEL-positive cells. These findings suggest that following parasympathetic nerve resection, mouse sublingual gland acinar cells undergo atrophy with a reduction size rather than cell death.
研究了接受副交感神经阻滞的小鼠舌下腺实质的形态学变化。对小鼠进行鼓索神经在其与舌神经汇合点附近的单侧切除。在1、2、3、5、10或20周后，处死小鼠并取出其舌下腺，进行光镜和电镜检查。切除后两周，处理侧腺体相邻小叶之间的间隙开始扩大，到10周时，其大小是未处理小鼠腺体间隙的10倍。切除后三周，小叶面积降至未处理小鼠腺体面积的约72%，腺泡面积降至约52%。然而，每组腺泡数量之间未见显著差异。电镜显示，处理侧腺体的分泌颗粒比未处理小鼠的腺体少，尽管大小无差异。处理侧腺体的小叶和未处理小鼠腺体的小叶均未含有许多TUNEL阳性细胞。这些发现表明，副交感神经切除后，小鼠舌下腺腺泡细胞发生萎缩，体积减小而非细胞死亡。
