Yano K, Lucas Z J
J Immunol. 1978 Feb;120(2):385-94.
To detect the cellular origins of alpha-lymphotoxin (alpha-LT), we cultured various subpopulations of human blood lymphocytes separated by erythrocyte-rosetting techniques with various mitogens. T cell-enriched subpopulations responded to PHA by increased 3H-thymidine uptake into DNA and large amounts of alpha-LT production. SPL and Con A-Sepharose stimulated DNA synthesis in T cell-enriched cultures if the macrophage content was greater than 1.5%; however, alpha-LT production was not induced by these two mitogens even when reconstituted with 10% macrophages. B and/or null cell-enriched populations severely depleted of T cells (less than 0.7% did not respond to PHA, SPL, or Con A-Sepharose. However, reconstitution to 5 or more percent in E-RFC allowed all three mitogens to stimulate DNA synthesis and alpha-LT production. The LT made by all cell populations 5 and 7 days after stimulation were equally neutralized by a heterologous antiserum to alpha-LT. These results show that human T and B and/or null cells, when appropriately stimulated, can produce alpha-LT.
为了检测α-淋巴细胞毒素（α-LT）的细胞来源，我们用红细胞玫瑰花结技术分离出人类血液淋巴细胞的不同亚群，并与各种有丝分裂原一起培养。富含T细胞的亚群对植物血凝素（PHA）有反应，表现为3H-胸腺嘧啶核苷摄取到DNA中的量增加以及大量α-LT的产生。如果巨噬细胞含量大于1.5%，则脾脏细胞（SPL）和刀豆蛋白A-琼脂糖（Con A-Sepharose）能刺激富含T细胞的培养物中的DNA合成；然而，即使加入10%的巨噬细胞进行重组，这两种有丝分裂原也不会诱导α-LT的产生。严重缺乏T细胞（少于0.7%）的富含B和/或裸细胞的群体对PHA、SPL或Con A-Sepharose无反应。然而，将E-玫瑰花结形成细胞（E-RFC）的比例恢复到5%或更高时，所有三种有丝分裂原都能刺激DNA合成和α-LT的产生。刺激后5天和7天，所有细胞群体产生的LT都能被抗α-LT的异源抗血清同等程度地中和。这些结果表明，人类T细胞、B细胞和/或裸细胞在受到适当刺激时可以产生α-LT。
