Walker S M, Lee S C, Lucas Z J
J Immunol. 1976 Mar;116(3):807-15.
Two different lymphotoxins synthesized by human blood lymphocytes stimulated with phytohemagglutin (PHA) are described. The two toxins are called alpha-LT and beta-LT relative to their elution order on gel chromatography. Their m.w. are 75,000 and 45,000 daltons, respectively. Both toxins appear as early as 7 hr after the addition of PHA, with the amount of beta-toxin exceeding that of alpha-LT initially. Both toxins are differentiated from a third toxin (adherent cell toxin, ACT) made by plastic-adherent cells without requiring mitogen-stimulation. Depletion of macrophages or B cells does not affect the synthesis of either lymphotoxin. Monospecific antisera were elicited to alpha-LT. Antisera elicited to beta-LT also neutralized alpha-LT but to a significantly lesser degree. Alpha-LT appears to be the lymphotoxin described by most other workers. Beta-LT is unstable at 37 degrees C which may explain why the low m.w. lymphotoxin has not been described previously.
本文描述了由植物血凝素（PHA）刺激的人血淋巴细胞合成的两种不同的淋巴毒素。根据它们在凝胶色谱上的洗脱顺序，这两种毒素分别称为α-LT和β-LT。它们的分子量分别为75,000和45,000道尔顿。早在加入PHA后7小时，两种毒素就出现了，最初β-毒素的量超过α-LT。这两种毒素与由塑料贴壁细胞产生的第三种毒素（贴壁细胞毒素，ACT）不同，后者无需有丝分裂原刺激。巨噬细胞或B细胞的耗竭不影响任何一种淋巴毒素的合成。制备了针对α-LT的单特异性抗血清。针对β-LT产生的抗血清也能中和α-LT，但程度明显较低。α-LT似乎是大多数其他研究者所描述的淋巴毒素。β-LT在37℃不稳定，这可能解释了为什么以前没有描述过低分子量的淋巴毒素。
