T细胞杂交瘤产生淋巴毒素和肿瘤坏死因子。
Production of lymphotoxin and tumour necrosis factor by a T-cell hybridoma.
作者信息
Kobayashi Y, Asada M, Osawa T
出版信息
Immunology. 1987 Feb;60(2):213-7.
It is known that two major cytotoxins, lymphotoxin (LT) and tumour necrosis factor (TNF), are produced by T cells and macrophages, respectively. We report in this paper that a human T-cell hybridoma, AC5-8, produces TNF in addition to LT depending on the kind of stimuli used. When phorbol myristate acetate (PMA) and concanavalin A (Con A) or PMA alone were used to induce AC5-8 cells to secrete a cytotoxin, we found that the cytotoxin secreted was antigenically indistinguishable from LT. On the other hand, when AC5-8 cells were activated by PMA and A23187, they secreted another cytotoxin which was antigenically indistinguishable from TNF, in addition to LT. These two cytotoxins could be separated by affinity chromatography on a column of Con A-Sepharose. A cytotoxin, which did not bind to the Con A-Sepharose column and showed about 70-95% of the total cytotoxic activity secreted from AC5-8 cells stimulated with PMA and A23187, was found to be antigenically identical to TNF. The other cytotoxin, which bound to the Con A-Sepharose column and showed only 5-30% of the total activity induced by PMA and A23187, was found to be antigenically identical to LT.
已知两种主要的细胞毒素，即淋巴毒素（LT）和肿瘤坏死因子（TNF），分别由T细胞和巨噬细胞产生。我们在本文中报告，人T细胞杂交瘤AC5 - 8根据所使用的刺激类型，除了产生LT外还产生TNF。当使用佛波酯肉豆蔻酸酯（PMA）和刀豆球蛋白A（Con A）或仅使用PMA诱导AC5 - 8细胞分泌细胞毒素时，我们发现分泌的细胞毒素在抗原性上与LT无法区分。另一方面，当AC5 - 8细胞被PMA和A23187激活时，除了LT外，它们还分泌另一种在抗原性上与TNF无法区分的细胞毒素。这两种细胞毒素可以通过Con A - Sepharose柱上的亲和层析分离。一种不与Con A - Sepharose柱结合且显示出在用PMA和A23187刺激的AC5 - 8细胞分泌的总细胞毒性活性中约70 - 95%的细胞毒素，被发现与TNF在抗原性上相同。另一种与Con A - Sepharose柱结合且仅显示出由PMA和A23187诱导的总活性的5 - 30%的细胞毒素，被发现与LT在抗原性上相同。
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