Farthing P M, Matear P, Cruchley A T
Department of Oral Pathology, London Hospital Medical College, England.
J Oral Pathol Med. 1992 Nov;21(10):451-5. doi: 10.1111/j.1600-0714.1992.tb00973.x.
Keratinocyte expression of the Class II major histocompatibility complex antigen HLADR, is seen in several inflammatory disorders of skin and mucosa, including lichen planus. The purpose of this study is to determine whether the distribution of Langerhans cells and their expression of CD4 in oral lichen planus is related to keratinocyte HLADR. The numbers of CD1- and CD4-positive Langerhans cells were compared in areas of keratinocyte HLADR and areas showing no expression in oral lichen planus and with normal oral mucosa. Cells were identified using an immunoalkaline phosphatase technique and numbers were expressed per mm epithelial surface length. In lichen planus, an increase both in the number of Langerhans cells and the numbers expressing CD4 were found in areas of keratinocyte HLADR expression compared with HLADR negative areas and with normal oral mucosa. There was no difference in the numbers of Langerhans cells or their expression of CD4 between HLADR-negative areas in LP and normal oral mucosa. These results show that the distribution of Langerhans cells is related to keratinocyte expression of HLADR and suggest that Langerhans cell entry may be enhanced in these areas. Whilst it is possible this enhancement is mediated by CD4/HLADR interaction, other molecules are also likely to be important in controlling Langerhans cell entry into oral mucosa.
角质形成细胞表达II类主要组织相容性复合体抗原HLADR，见于包括扁平苔藓在内的多种皮肤和黏膜炎症性疾病。本研究的目的是确定口腔扁平苔藓中朗格汉斯细胞的分布及其CD4表达是否与角质形成细胞HLADR有关。比较了口腔扁平苔藓中角质形成细胞HLADR区域和无表达区域以及正常口腔黏膜中CD1阳性和CD4阳性朗格汉斯细胞的数量。使用免疫碱性磷酸酶技术识别细胞，数量以每毫米上皮表面长度表示。在扁平苔藓中，与HLADR阴性区域和正常口腔黏膜相比，在角质形成细胞HLADR表达区域发现朗格汉斯细胞数量和表达CD4的细胞数量均增加。扁平苔藓中HLADR阴性区域与正常口腔黏膜之间朗格汉斯细胞数量或其CD4表达没有差异。这些结果表明朗格汉斯细胞的分布与角质形成细胞HLADR的表达有关，并提示在这些区域朗格汉斯细胞的进入可能增强。虽然这种增强可能是由CD4/HLADR相互作用介导的，但其他分子在控制朗格汉斯细胞进入口腔黏膜方面也可能很重要。
