Yahong Chen, Ruxiu Cai
Department of Chemistry, Wuhan University, Wuhan 430072, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2003 Dec;59(14):3357-62. doi: 10.1016/s1386-1425(03)00141-0.
A highly sensitive spectrofluorimetric method for the determination of L-cysteine based on its inhibitory action on hemoglobin (Hb) activity was developed. The optimal conditions were studied. A linear calibration graph was obtained over the range 2.00 x 10(-7)-1.50 x 10(-6) mol l(-1) for L-cysteine. The relative standard derivation was 1.30% at L-cysteine concentration of 8.00 x 10(-7) mol l(-1) (n=11). Further experimental results revealed that the inhibition of L-cysteine on this system was of the competitive type. The method was applied to determine L-cysteine in protein hydrolysate and cystine electrolyte samples with satisfactory results.
基于L-半胱氨酸对血红蛋白（Hb）活性的抑制作用，开发了一种高灵敏度的荧光分光光度法用于测定L-半胱氨酸。研究了最佳条件。L-半胱氨酸在2.00×10⁻⁷ - 1.50×10⁻⁶ mol·L⁻¹范围内获得线性校准曲线。L-半胱氨酸浓度为8.00×10⁻⁷ mol·L⁻¹时（n = 11），相对标准偏差为1.30%。进一步的实验结果表明，L-半胱氨酸对该体系的抑制作用为竞争型。该方法用于测定蛋白质水解物和胱氨酸电解质样品中的L-半胱氨酸，结果令人满意。
