基于多酶氧化还原体系对芦丁的激活作用的高灵敏荧光光度法测定芦丁。

Highly sensitive spectrofluorimetic determination of rutin based on its activated effect on a multi-enzyme redox system.

机构信息

Department of Chemistry, Zhoukou Normal University, Zhoukou, 466000, People's Republic of China.

出版信息

Luminescence. 2012 Jan-Feb;27(1):59-62. doi: 10.1002/bio.1325. Epub 2011 Jul 11.

Abstract

A highly sensitive and simple spectrofluorimetric method for the determination of rutin, based on its activated effect on a haemoglobin-catalysed reaction, was developed. Under optimum conditions, the concentration of rutin was linear, with decreased fluorescence (ΔF) of the system under optimal experimental conditions. The calibration graph was linear in the range 1.0 × 10(-7) -3.0 × 10(-5) mol/L, with a detection limit of 7.0 × 10(-8) mol/L. The relative standard deviation (RSD) was 3.26% for 11 determinations of 1.0 × 10(-5) mol/L. This method was used for the determination of rutin in pharmaceuticals with satisfactory results.

摘要

建立了一种基于血红蛋白催化反应的芦丁高灵敏度和简单的荧光分光光度法。在最佳条件下，芦丁的浓度与体系的荧光强度（ΔF）呈线性关系，在最佳实验条件下，荧光强度降低。校准曲线在 1.0×10（-7）-3.0×10（-5）mol/L 范围内呈线性，检测限为 7.0×10（-8）mol/L。在 1.0×10（-5）mol/L 下测定 11 次，相对标准偏差（RSD）为 3.26%。该方法用于测定药物中的芦丁，结果令人满意。