Department of Chemistry, Zhoukou Normal University, Zhoukou, 466000, People's Republic of China.
Luminescence. 2011 Nov-Dec;26(6):477-80. doi: 10.1002/bio.1255. Epub 2010 Oct 20.
A highly sensitive and simple spectrofluorimetric method for the determination of tiopronin based on its inhibitory effect on the hemoglobin-catalyzed reaction of H(2)O(2) and L-tyrosine was developed. The concentration of tiopronin is linear with decreased fluorescence (ΔF) of the system under the optimal experimental conditions. The calibration graph is linear in the range 1.23 × 10(-8) to 3.06 × 10(-5) mol L(-1) with a detection limit of 6.13 × 10(-9) mol L(-1). The relative standard deviation was 4.38% for 11 determinations of 6.13 × 10(-6) mol L(-1). This method can be used for the determination of tiopronin in pharmaceuticals with satisfactory results.
建立了一种基于血红蛋白催化 H 2 O 2 和 L-酪氨酸反应被抑制的作用来测定巯丁二酸的高灵敏、简单的荧光光谱法。在最佳实验条件下，随着体系荧光（ΔF）的降低，巯丁二酸的浓度与线性相关。校准曲线在 1.23×10^-8 至 3.06×10^-5 mol·L^-1 范围内呈线性，检测限为 6.13×10^-9 mol·L^-1。对 6.13×10^-6 mol·L^-1 进行 11 次测定，相对标准偏差为 4.38%。该方法可用于药物中巯丁二酸的测定，结果令人满意。
